- PA5-22377 - Invitrogen Antibodies ALDH1A2 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of ALDH1A2 using Mouse tissue extracts (50 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with an ALDH1A2 polyclonal antibody (Product # PA5-22377) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ALDH1A2 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ALDH1A2 Polyclonal Antibody (Product # PA5-22377) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ALDH1A2 Polyclonal Antibody (Product # PA5-22377). Sample (30 µg of whole cell lysate). Lane A: IMR32. 10% SDS PAGE. ALDH1A2 Polyclonal Antibody (Product # PA5-22377) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ALDH1A2 antibody detects ALDH1A2 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 antibody ALDH1A2 Polyclonal Antibody (Product # PA5-22377) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ALDH1A2 Polyclonal Antibody (Product # PA5-22377). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 7.5% SDS PAGE. ALDH1A2 Polyclonal Antibody (Product # PA5-22377) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), HEK-293 (Lane 4) and tissue extracts (30 µg lysate) of Mouse Uterus (Lane 5) and Mouse Brain (Lane 6). The blot was probed with Anti-ALDH1A2 Polyclonal Antibody (Product # PA5-22377, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 56 kDa band corresponding to ALDH1A2 was detected in K-562, HEK-293 and Mouse Uterus and not in HeLa, MCF7 and Mouse Brain which are reported negative for ALDH1A2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of ALDH1A2 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ALDH1A2 Polyclonal Antibody (Product # PA5-22377) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing membranous localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry (Paraffin) analysis of ALDH1A2 was performed in paraffin-embedded mouse testis tissue using ALDH1A2 Polyclonal Antibody (Product # PA5-22377) at a dilution of 1:500.

PA5-22377