PA5-30288

antibody from Invitrogen Antibodies

Targeting: ALDH1A2 RALDH2

Antibody data

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Validation data

Product number: PA5-30288 - Provider product page
Provider: Invitrogen Antibodies
Product name: ALDH1A2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: MCF-7, mouse testis, rat testis. Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (81%), Japanese Medaka (81%), Xenopus laevis (88%), Dog (99%), Chicken (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.73 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ALDH1A2 protein structure - PA5-30288 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ALDH1A2 using Mouse tissue extracts (50 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with an ALDH1A2 polyclonal antibody (Product # PA5-30288) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ALDH1A2 using 50 µg of mouse liver lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an ALDH1A2 polyclonal antibody (Product # PA5-30288) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ALDH1A2 using 30 µg of MCF-7 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ALDH1A2 polyclonal antibody (Product # PA5-30288) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ALDH1A2 in whole cell extracts (30 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with ALDH1A2 Polyclonal antibody (Product # PA5-30288) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ALDH1A2 was performed by separating 50 µg of mouse tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ALDH1A2 Polyclonal Antibody (Product # PA5-30288) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ALDH1A2 Polyclonal Antibody (Product # PA5-30288). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A2 Polyclonal Antibody (Product # PA5-30288) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ALDH1A2 was performed by separating 50 µg of rat tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ALDH1A2 Polyclonal Antibody (Product # PA5-30288) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ALDH1A2 was performed by separating 30 µg of whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ALDH1A2 Polyclonal Antibody (Product # PA5-30288) at a dilution of 1:1000.