H00001312-M01

antibody from Abnova Corporation

Targeting: COMT

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001312-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001312-M01, RRID:AB_425377

Product name

COMT monoclonal antibody (M01), clone 1G4-1A1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant COMT.

Antigen sequence

MNVGDKKGKIVDAVIQEHQPSVLLELGAYCGYSAV
RMARLLSPGARLITIEINPDCAAITQRMVDFAGMK
DKVTLVVGASQDIIPQLKKKYDVDTLDMVFLDHWK
DRYLPDTLLLEECGLLRKGTVLLADNVICPGAPDF
LAHVRGSSCFECTHYQSFLEYREVVDGLEKAIYKG
PGSEAGP


Isotype

IgG

Antibody clone number

1G4-1A1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

COMT monoclonal antibody (M01), clone 1G4-1A1 Western Blot analysis of COMT expression in A-431 ( Cat # L015V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of COMT expression in transfected 293T cell line by COMT monoclonal antibody (M01), clone 1G4-1A1.Lane 1: COMT transfected lysate(24.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged COMT is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol