Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-77920 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COMT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Molt-4, mouse spleen Predicted Reactivity: Rat (82%), Dog (81%), Pig (82%), Rhesus Monkey (93%), Bovine (81%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.32 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COMT in mouse spleen using 50 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with COMT polyclonal antibody (Product # PA5-77920) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COMT in Molt-4 cells using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with COMT polyclonal antibody (Product # PA5-77920) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COMT Polyclonal Antibody (Product # PA5-77920). Sample (30 µg of whole cell lysate). Lane A: Molt-4. 12% SDS PAGE. COMT Polyclonal Antibody (Product # PA5-77920) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COMT Polyclonal Antibody detects COMT protein by Western blot analysis. A. 50 µg mouse spleen lysate/extract.12 % SDS-PAGE. COMT Polyclonal Antibody (Product # PA5-77920) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA detection of COMT using COMT Polyclonal Antibody (Product # PA5-77920) for capture at a concentration of 2.5 µg/mL and a COMT Polyclonal Antibody for detection at a concentration of 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COMT Polyclonal Antibody detects COMT protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. COMT Polyclonal Antibody (Product # PA5-77920) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.