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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-88877 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COMT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VGDKKGKIVD AVIQEHQPSV LLELGAYCGY SAVRMARLLS PGARLITIEI NPDCAAITQR MVDFAGVKDK VTLVVGASQD IIPQLKKKYD VDTLDMVFLD HWKDRYLPDT LLLEECGLLR KGTVLLADNV ICPGAPDFLA HVRGSSCFEC THYQSFLEYR EVVDGLEKAI YKGPGSEAGP; Positive Samples: MCF-7, HT-29, THP-1, A-431, HepG2, Mouse liver; Cellular Location: Cell membrane, Cytoplasm, Extracellular side, Single-pass type II membrane protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.43 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using COMT Polyclonal antibody (Product # PA5-88877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COMT in extracts of various cell lines using COMT Polyclonal Antibody (Product # PA5-88877) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of COMT was performed in 200 µg extracts of MCF-7 cells using COMT Polyclonal Antibody (Product # PA5-88877). Western blot was performed from the immunoprecipitate using COMT Polyclonal Antibody.
