Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-47679 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 5T4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse 5T4 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human 5T4 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47679) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for 5T4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 5T4 in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in 5T4 polyclonal antibody (Product # PA5-47679) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for 5T4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of 5T4 in immersion fixed paraffin-embedded sections of human placenta. Samples were incubated in 5T4 polyclonal antibody (Product # PA5-47679) using a dilution of 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent‚Basic. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of 5T4 in immersion fixed paraffin-embedded sections of human placenta. Samples were incubated in 5T4 polyclonal antibody (Product # PA5-47679) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.