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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- STJ99093 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-SIRT2 antibody [1D4-H11-H11] (STJ99093)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Nad-Dependent Protein Deacetylase Sirtuin-2 is suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry research applications.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of SirT2 in PC-12, Rat Brain and Mouse Brain cell lysates using SirT2 mouse mAb (1:1000 diluted). Predicted band size: 39, 43KDa. Observed band size:39, 43KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at-20℃ and using SirT2 (200474, dilution 1:50) mouse mAb (green) and Histone H3.1 (Phospho-Ser10) (310045, dilution 1:200) Rabbit pAb (red).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
