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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [2]
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- Product number
- AF265 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat Angiogenin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Angiogenin in direct ELISAs and Western blots.
- Reactivity
- Human, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q53X86- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An essential role of PRMT1-mediated EGFR methylation in EGFR activation by ribonuclease 5.
Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C.
Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4.
A neuroprotective role for angiogenin in models of Parkinson's disease.
Wang WJ, Hsu JM, Wang YN, Lee HH, Yamaguchi H, Liao HW, Hung MC
American journal of cancer research 2019;9(1):180-185
American journal of cancer research 2019;9(1):180-185
Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C.
Selitsky SR, Baran-Gale J, Honda M, Yamane D, Masaki T, Fannin EE, Guerra B, Shirasaki T, Shimakami T, Kaneko S, Lanford RE, Lemon SM, Sethupathy P
Scientific reports 2015 Jan 8;5:7675
Scientific reports 2015 Jan 8;5:7675
Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4.
Burger-van Paassen N, Loonen LM, Witte-Bouma J, Korteland-van Male AM, de Bruijn AC, van der Sluis M, Lu P, Van Goudoever JB, Wells JM, Dekker J, Van Seuningen I, Renes IB
PloS one 2012;7(6):e38798
PloS one 2012;7(6):e38798
A neuroprotective role for angiogenin in models of Parkinson's disease.
Steidinger TU, Standaert DG, Yacoubian TA
Journal of neurochemistry 2011 Feb;116(3):334-41
Journal of neurochemistry 2011 Feb;116(3):334-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat Angiogenin by Western Blot. Western blot shows human plasma and rat plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiogenin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat Angiogenin by Simple WesternTM. Simple Western lane view shows human plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for Angiogenin at approximately 20 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
