Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-78662 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEIL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: U87-MG, U87-MG nuclear extract Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.88 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NEIL2 in U87-MG cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with NEIL2 polyclonal antibody (Product # PA5-78662) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NEIL2 Polyclonal Antibody (Product # PA5-78662). U87-MG whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEIL2 Polyclonal Antibody (Product # PA5-78662) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NEIL2 Polyclonal Antibody (Product # PA5-78662). U87-MG whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEIL2 Polyclonal Antibody (Product # PA5-78662) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NEIL2 was performed in U87-MG cells fixed in ice-cold MeOH for 5 min. Green: NEIL2 Polyclonal Antibody (Product # PA5-78662) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.