PA5-78662

NEIL2 antibody from Invitrogen Antibodies

FLJ31644, MGC2832, MGC4505, NEH2

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Provider product page for PA5-78662

Antibody Data

Product number

PA5-78662

Provider

Invitrogen Antibodies

Product name

NEIL2 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.88 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NEIL2 in U87-MG cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with NEIL2 polyclonal antibody (Product # PA5-78662) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NEIL2 Polyclonal Antibody (Product # PA5-78662). U87-MG whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEIL2 Polyclonal Antibody (Product # PA5-78662) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of NEIL2 was performed in U87-MG cells fixed in ice-cold MeOH for 5 min. Green: NEIL2 Polyclonal Antibody (Product # PA5-78662) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.