PA5-78662

antibody from Invitrogen Antibodies

Targeting: NEIL2 FLJ31644, MGC2832, MGC4505, NEH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78662

Provider

Invitrogen Antibodies

Product name

NEIL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: U87-MG, U87-MG nuclear extract

Concentration

0.88 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NEIL2 in U87-MG cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with NEIL2 polyclonal antibody (Product # PA5-78662) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NEIL2 Polyclonal Antibody (Product # PA5-78662). U87-MG whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEIL2 Polyclonal Antibody (Product # PA5-78662) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NEIL2 Polyclonal Antibody (Product # PA5-78662). U87-MG whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEIL2 Polyclonal Antibody (Product # PA5-78662) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of NEIL2 was performed in U87-MG cells fixed in ice-cold MeOH for 5 min. Green: NEIL2 Polyclonal Antibody (Product # PA5-78662) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.