PA5-77297
antibody from Invitrogen Antibodies
Targeting: CACNA1C
CACH2, CACN2, CACNL1A1, Cav1.2, CCHL1A1, LQT8, TS
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-77297 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CaV1.2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references Electrophysiologic and molecular mechanisms of a frameshift NPPA mutation linked with familial atrial fibrillation.
Menon A, Hong L, Savio-Galimberti E, Sridhar A, Youn SW, Zhang M, Kor K, Blair M, Kupershmidt S, Darbar D
Journal of molecular and cellular cardiology 2019 Jul;132:24-35
Journal of molecular and cellular cardiology 2019 Jul;132:24-35
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CaV1.2-transfected Xenopus oocytes (lane 1) and non-transfected oocytes lysates (lane 2) with CaV1.2 polyclonal antibody (Product # PA5-77297) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CaV1.2 in rat brain membranes using a CaV1.2 polyclonal antibody (Product # PA5-77297) diluted 1:200 (lane 1) or using CaV1.2 polyclonal antibody preincubated with a control peptide antigen (lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CaV1.2 in mouse cerebellum. A) Samples were probed with CaV1.2 polyclonal antibody (Product # PA5-77297) and incubated with calbindin 28K (green). B) Astrocyte stained image. C) Merged image of Panels A and B.