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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Flow cytometry [2]
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- Product number
- PA5-109279 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KV1.5 (KCNA5) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: PANC-1, LOVO, SKOV-3, rat skeletal muscle tissue, rat heart tissue.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KCNA5 in PANC-1 cell lysate. Samples were incubated with KCNA5 polyclonal antibody (Product # PA5-109279), at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KCNA5 in SKOV-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KCNA5 polyclonal antibody (Product # PA5-109279), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KCNA5 in PANC-1 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KCNA5 polyclonal antibody (Product # PA5-109279), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KCNA5 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KCNA5 polyclonal antibody (Product # PA5-109279), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of KCNA5 in PANC-1 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with KCNA5 polyclonal antibody (Product # PA5-109279) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of KCNA5 in PANC-1 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with KCNA5 polyclonal antibody (Product # PA5-109279) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
