15090-1-AP
antibody from Invitrogen Antibodies
Targeting: ALDH3A2
ALDH10, FALDH, SLS
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 15090-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH3A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MELEVRRVR QAFLSGRSRP LRFRLQQLEA LRRMVQEREK DILTAIAADL CKSEFNVYSQ EVITVLGEID FMLENLPEWV TAKPVKKNVL TMLDEAYIQP QPLGVVLIIG AWNYPFVLTI QPLIGAIAAG NAVIIKPSEL SENTAKILAK LLPQYLDQDL YIVINGGVEE TTELLKQRFD HIFYTGNTAV GKIVMEAAAK HLTPVTLELG GKSPCYIDKD CDLDIVCRRI TWGKYMNCGQ TCIAPDYILC EASLQNQIVW KIKETVKEFY GENIKESPDY ERIINLRHFK RILSLLEGQK IAFGGETDEA TRYIA (1-314 aa encoded by BC002430)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.28 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using ALDH3A2 antibody 15090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug ALDH3A2 antibody (15090-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ALDH3A2 (IP:15090-1-AP, 4ug; Detection:15090-1-AP 1:1000) with HEK-293 cells lysate 3000ug.