Antibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 20854-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20854-1-AP, RRID:AB_10693629
- Product name
- NSUN2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated NSUN2 antibody (Cat. #20854-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references NSUN2 modified by SUMO-2/3 promotes gastric cancer progression and regulates mRNA m5C methylation.
Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC.
Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.
RNA methyltransferase NSUN2 promotes hypopharyngeal squamous cell carcinoma proliferation and migration by enhancing TEAD1 expression in an m5C-dependent manner.
Neuronal Nsun2 deficiency produces tRNA epitranscriptomic alterations and proteomic shifts impacting synaptic signaling and behavior.
Disease Activity-Associated Alteration of mRNA m5 C Methylation in CD4+ T Cells of Systemic Lupus Erythematosus.
CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.
Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures.
Epitranscriptomic 5-Methylcytosine Profile in PM2.5-induced Mouse Pulmonary Fibrosis.
5-methylcytosine modification of an Epstein-Barr virus noncoding RNA decreases its stability.
Multiple links between 5-methylcytosine content of mRNA and translation.
NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs.
NOP2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL6 in gallbladder carcinoma.
Genome-wide identification of mRNA 5-methylcytosine in mammals.
Cytosine-5 RNA methylation links protein synthesis to cell metabolism.
Extensive Epitranscriptomic Methylation of A and C Residues on Murine Leukemia Virus Transcripts Enhances Viral Gene Expression.
5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.
Epitranscriptomic Addition of m5C to HIV-1 Transcripts Regulates Viral Gene Expression.
5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m5C reader.
The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.
Compound Heterozygosity for Y Box Proteins Causes Sterility Due to Loss of Translational Repression.
tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells.
Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.
Identification of direct targets and modified bases of RNA cytosine methyltransferases.
The mouse cytosine-5 RNA methyltransferase NSun2 is a component of the chromatoid body and required for testis differentiation.
Whole exome sequencing identifies a splicing mutation in NSUN2 as a cause of a Dubowitz-like syndrome.
Hu Y, Chen C, Tong X, Chen S, Hu X, Pan B, Sun X, Chen Z, Shi X, Hu Y, Shen X, Xue X, Lu M
Cell death & disease 2021 Sep 9;12(9):842
Cell death & disease 2021 Sep 9;12(9):842
Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC.
Ohshiro T, Konno M, Asai A, Komoto Y, Yamagata A, Doki Y, Eguchi H, Ofusa K, Taniguchi M, Ishii H
Scientific reports 2021 Sep 29;11(1):19304
Scientific reports 2021 Sep 29;11(1):19304
Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.
Dai W, Li A, Yu NJ, Nguyen T, Leach RW, Wühr M, Kleiner RE
Nature chemical biology 2021 Nov;17(11):1178-1187
Nature chemical biology 2021 Nov;17(11):1178-1187
RNA methyltransferase NSUN2 promotes hypopharyngeal squamous cell carcinoma proliferation and migration by enhancing TEAD1 expression in an m5C-dependent manner.
Chen L, Ding J, Wang B, Chen X, Ying X, Yu Z, Dong P
Experimental cell research 2021 Jul 15;404(2):112664
Experimental cell research 2021 Jul 15;404(2):112664
Neuronal Nsun2 deficiency produces tRNA epitranscriptomic alterations and proteomic shifts impacting synaptic signaling and behavior.
Blaze J, Navickas A, Phillips HL, Heissel S, Plaza-Jennings A, Miglani S, Asgharian H, Foo M, Katanski CD, Watkins CP, Pennington ZT, Javidfar B, Espeso-Gil S, Rostandy B, Alwaseem H, Hahn CG, Molina H, Cai DJ, Pan T, Yao WD, Goodarzi H, Haghighi F, Akbarian S
Nature communications 2021 Aug 13;12(1):4913
Nature communications 2021 Aug 13;12(1):4913
Disease Activity-Associated Alteration of mRNA m5 C Methylation in CD4+ T Cells of Systemic Lupus Erythematosus.
Guo G, Wang H, Shi X, Ye L, Yan K, Chen Z, Zhang H, Jin Z, Xue X
Frontiers in cell and developmental biology 2020;8:430
Frontiers in cell and developmental biology 2020;8:430
CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.
Fang L, Wang W, Li G, Zhang L, Li J, Gan D, Yang J, Tang Y, Ding Z, Zhang M, Zhang W, Deng D, Song Z, Zhu Q, Cui H, Hu Y, Chen W
Molecular systems biology 2020 Nov;16(11):e10025
Molecular systems biology 2020 Nov;16(11):e10025
Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures.
Begik O, Lucas MC, Liu H, Ramirez JM, Mattick JS, Novoa EM
Genome biology 2020 May 7;21(1):97
Genome biology 2020 May 7;21(1):97
Epitranscriptomic 5-Methylcytosine Profile in PM2.5-induced Mouse Pulmonary Fibrosis.
Han X, Liu H, Zhang Z, Yang W, Wu C, Liu X, Zhang F, Sun B, Zhao Y, Jiang G, Yang YG, Ding W
Genomics, proteomics & bioinformatics 2020 Feb;18(1):41-51
Genomics, proteomics & bioinformatics 2020 Feb;18(1):41-51
5-methylcytosine modification of an Epstein-Barr virus noncoding RNA decreases its stability.
Henry BA, Kanarek JP, Kotter A, Helm M, Lee N
RNA (New York, N.Y.) 2020 Aug;26(8):1038-1048
RNA (New York, N.Y.) 2020 Aug;26(8):1038-1048
Multiple links between 5-methylcytosine content of mRNA and translation.
Schumann U, Zhang HN, Sibbritt T, Pan A, Horvath A, Gross S, Clark SJ, Yang L, Preiss T
BMC biology 2020 Apr 15;18(1):40
BMC biology 2020 Apr 15;18(1):40
NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs.
Van Haute L, Lee SY, McCann BJ, Powell CA, Bansal D, Vasiliauskaitė L, Garone C, Shin S, Kim JS, Frye M, Gleeson JG, Miska EA, Rhee HW, Minczuk M
Nucleic acids research 2019 Sep 19;47(16):8720-8733
Nucleic acids research 2019 Sep 19;47(16):8720-8733
NOP2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL6 in gallbladder carcinoma.
Gao Y, Wang Z, Zhu Y, Zhu Q, Yang Y, Jin Y, Zhang F, Jiang L, Ye Y, Li H, Zhang Y, Liang H, Xiang S, Miao H, Liu Y, Hao Y
Cancer science 2019 Nov;110(11):3510-3519
Cancer science 2019 Nov;110(11):3510-3519
Genome-wide identification of mRNA 5-methylcytosine in mammals.
Huang T, Chen W, Liu J, Gu N, Zhang R
Nature structural & molecular biology 2019 May;26(5):380-388
Nature structural & molecular biology 2019 May;26(5):380-388
Cytosine-5 RNA methylation links protein synthesis to cell metabolism.
Gkatza NA, Castro C, Harvey RF, Heiß M, Popis MC, Blanco S, Bornelöv S, Sajini AA, Gleeson JG, Griffin JL, West JA, Kellner S, Willis AE, Dietmann S, Frye M
PLoS biology 2019 Jun;17(6):e3000297
PLoS biology 2019 Jun;17(6):e3000297
Extensive Epitranscriptomic Methylation of A and C Residues on Murine Leukemia Virus Transcripts Enhances Viral Gene Expression.
Courtney DG, Chalem A, Bogerd HP, Law BA, Kennedy EM, Holley CL, Cullen BR
mBio 2019 Jun 11;10(3)
mBio 2019 Jun 11;10(3)
5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.
Chen X, Li A, Sun BF, Yang Y, Han YN, Yuan X, Chen RX, Wei WS, Liu Y, Gao CC, Chen YS, Zhang M, Ma XD, Liu ZW, Luo JH, Lyu C, Wang HL, Ma J, Zhao YL, Zhou FJ, Huang Y, Xie D, Yang YG
Nature cell biology 2019 Aug;21(8):978-990
Nature cell biology 2019 Aug;21(8):978-990
Epitranscriptomic Addition of m5C to HIV-1 Transcripts Regulates Viral Gene Expression.
Courtney DG, Tsai K, Bogerd HP, Kennedy EM, Law BA, Emery A, Swanstrom R, Holley CL, Cullen BR
Cell host & microbe 2019 Aug 14;26(2):217-227.e6
Cell host & microbe 2019 Aug 14;26(2):217-227.e6
5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m5C reader.
Yang X, Yang Y, Sun BF, Chen YS, Xu JW, Lai WY, Li A, Wang X, Bhattarai DP, Xiao W, Sun HY, Zhu Q, Ma HL, Adhikari S, Sun M, Hao YJ, Zhang B, Huang CM, Huang N, Jiang GB, Zhao YL, Wang HL, Sun YP, Yang YG
Cell research 2017 May;27(5):606-625
Cell research 2017 May;27(5):606-625
The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.
Simsek D, Tiu GC, Flynn RA, Byeon GW, Leppek K, Xu AF, Chang HY, Barna M
Cell 2017 Jun 1;169(6):1051-1065.e18
Cell 2017 Jun 1;169(6):1051-1065.e18
Compound Heterozygosity for Y Box Proteins Causes Sterility Due to Loss of Translational Repression.
Snyder E, Soundararajan R, Sharma M, Dearth A, Smith B, Braun RE
PLoS genetics 2015 Dec;11(12):e1005690
PLoS genetics 2015 Dec;11(12):e1005690
tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells.
Okamoto M, Fujiwara M, Hori M, Okada K, Yazama F, Konishi H, Xiao Y, Qi G, Shimamoto F, Ota T, Temme A, Tatsuka M
PLoS genetics 2014 Sep;10(9):e1004639
PLoS genetics 2014 Sep;10(9):e1004639
Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.
Khoddami V, Cairns BR
Nature protocols 2014 Feb;9(2):337-61
Nature protocols 2014 Feb;9(2):337-61
Identification of direct targets and modified bases of RNA cytosine methyltransferases.
Khoddami V, Cairns BR
Nature biotechnology 2013 May;31(5):458-64
Nature biotechnology 2013 May;31(5):458-64
The mouse cytosine-5 RNA methyltransferase NSun2 is a component of the chromatoid body and required for testis differentiation.
Hussain S, Tuorto F, Menon S, Blanco S, Cox C, Flores JV, Watt S, Kudo NR, Lyko F, Frye M
Molecular and cellular biology 2013 Apr;33(8):1561-70
Molecular and cellular biology 2013 Apr;33(8):1561-70
Whole exome sequencing identifies a splicing mutation in NSUN2 as a cause of a Dubowitz-like syndrome.
Martinez FJ, Lee JH, Lee JE, Blanco S, Nickerson E, Gabriel S, Frye M, Al-Gazali L, Gleeson JG
Journal of medical genetics 2012 Jun;49(6):380-5
Journal of medical genetics 2012 Jun;49(6):380-5
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20854-1-AP(NSUN2 antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using NSUN2 antibody 20854-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 20854-1-AP(NSUN2 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 20854-1-AP(NSUN2 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue