20854-1-AP

antibody from Proteintech Group

Targeting: NSUN2 FLJ20303, Misu, MRT5, TRM4

Provider product page for 20854-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [63]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 20854-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20854-1-AP, RRID:AB_10693629
Product name: NSUN2 antibody
Antibody type: Polyclonal
Description: KD/KO validated NSUN2 antibody (Cat. #20854-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NSUN2 protein structure - 20854-1-AP shown in red.

Submitted references LncRNA HOST2 promotes NSUN2-mediated breast cancer progression via interaction with ELAVL1.

Wang X, Liu D, Hua K, Fang L
Cellular signalling 2024 May;117:111112

RNA m(5)C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer.

Liu X, Wei Q, Yang C, Zhao H, Xu J, Mobet Y, Luo Q, Yang D, Zuo X, Chen N, Yang Y, Li L, Wang W, Yu J, Xu J, Liu T, Yi P
Experimental & molecular medicine 2024 Mar;56(3):600-615

NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA stabilization.

Zou S, Huang Y, Yang Z, Zhang J, Meng M, Zhang Y, Feng J, Sun R, Li W, Wang W, López JG, Fang L
Clinical and translational medicine 2024 Mar;14(3):e1621

Suppression of NSUN2 enhances the sensitivity to chemosensitivity and inhibits proliferation by mediating cell apoptosis in gastric cancer.

Shen X, Sun H, Shu S, Tang W, Yuan Y, Su H, Li Y, Fan H
Pathology, research and practice 2024 Jan;253:154986

Epigenetic addition of m(5)C to HBV transcripts promotes viral replication and evasion of innate antiviral responses.

Ding S, Liu H, Liu L, Ma L, Chen Z, Zhu M, Liu L, Zhang X, Hao H, Zuo L, Yang J, Wu X, Zhou P, Huang F, Zhu F, Guan W
Cell death & disease 2024 Jan 12;15(1):39

m5C modification of LINC00324 promotes angiogenesis in glioma through CBX3/VEGFR2 pathway.

Pan A, Xue Y, Ruan X, Dong W, Wang D, Liu Y, Liu L, Lin Y, E T, Lin H, Xu H, Liu X, Wang P
International journal of biological macromolecules 2024 Feb;257(Pt 1):128409

ALYREF-JunD-SLC7A5 axis promotes pancreatic ductal adenocarcinoma progression through epitranscriptome-metabolism reprogramming and immune evasion.

Meng Q, Xie Y, Sun K, He L, Wu H, Zhang Q, Liang T
Cell death discovery 2024 Feb 24;10(1):97

5-Methylcytosine transferase NSUN2 drives NRF2-mediated ferroptosis resistance in non-small cell lung cancer.

Chen Y, Jiang Z, Zhang C, Zhang L, Chen H, Xiao N, Bai L, Liu H, Wan J
The Journal of biological chemistry 2024 Apr;300(4):106793

NSUN2 relies on ALYREF to regulate Nrf2-mediated oxidative stress and alleviate Dox-induced liver injury.

Huang Y, Li X, Wei L, Ma S, Ma L, Zan Y, He X, Tang Y, Ding Y
Biology direct 2024 Apr 29;19(1):32

Upregulation of LRRC8A by m(5)C modification-mediated mRNA stability suppresses apoptosis and facilitates tumorigenesis in cervical cancer.

Chen Y, Zuo X, Wei Q, Xu J, Liu X, Liu S, Wang H, Luo Q, Wang Y, Yang Y, Zhao H, Xu J, Liu T, Yi P
International journal of biological sciences 2023;19(2):691-704

NOP2/Sun RNA methyltransferase 2 is a potential pan-cancer prognostic biomarker and is related to immunity.

Zheng L, Li M, Wei J, Chen S, Xue C, Duan Y, Tang F, Li G, Xiong W, She K, Deng H, Zhou M
PloS one 2023;18(9):e0292212

RNA cytosine methyltransferase NSUN5 promotes protein synthesis and tumorigenic phenotypes in glioblastoma.

Zhou J, Kong YS, Vincent KM, Dieters-Castator D, Bukhari AB, Glubrecht D, Liu RZ, Quilty D, Findlay SD, Huang X, Xu Z, Yang RZ, Zhang L, Tang E, Lajoie G, Eisenstat DD, Gamper AM, Fahlman R, Godbout R, Postovit LM, Fu Y
Molecular oncology 2023 Sep;17(9):1763-1783

Vitamin D3 regulates NSUN2 expression and inhibits melanoma cell proliferation and migration.

Wang L, Zhang Q, Wang J, Lu H, Zeng W, Zhang T
Molecular diversity 2023 Sep 9;

RNA 5-Methylcytosine regulators are associated with cell adhesion and predict prognosis of endometrial cancer.

Yang S, Luo Y, Zhou D, Xiang J, Xi X
Translational cancer research 2023 Oct 31;12(10):2556-2571

NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance.

Chen T, Xu ZG, Luo J, Manne RK, Wang Z, Hsu CC, Pan BS, Cai Z, Tsai PJ, Tsai YS, Chen ZZ, Li HY, Lin HK
Cell metabolism 2023 Oct 3;35(10):1782-1798.e8

NSUN2-mediated m(5) C RNA methylation dictates retinoblastoma progression through promoting PFAS mRNA stability and expression.

Zuo S, Li L, Wen X, Gu X, Zhuang A, Li R, Ye F, Ge S, Fan X, Fan J, Chai P, Lu L
Clinical and translational medicine 2023 May;13(5):e1273

RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation.

Kim YA, Siddiqui T, Blaze J, Cosacak MI, Winters T, Kumar A, Tein E, Sproul AA, Teich AF, Bartolini F, Akbarian S, Kizil C, Hargus G, Santa-Maria I
Acta neuropathologica 2023 Jan;145(1):29-48

NSUN2 alleviates doxorubicin-induced myocardial injury through Nrf2-mediated antioxidant stress.

Wang Y, Zan Y, Huang Y, Peng X, Ma S, Ren J, Li X, Wei L, Wang X, Yuan Y, Tang J, Zhan Z, Wang Z, Ding Y
Cell death discovery 2023 Feb 4;9(1):43

m(5)C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization.

Wang N, Chen RX, Deng MH, Wei WS, Zhou ZH, Ning K, Li YH, Li XD, Ye YL, Wen JH, Dong B, Zhang XP, Liu ZW, Zhou FJ
Cell death & disease 2023 Feb 18;14(2):139

Nsun2 coupling with RoRγt shapes the fate of Th17 cells and promotes colitis.

Yang WL, Qiu W, Zhang T, Xu K, Gu ZJ, Zhou Y, Xu HJ, Yang ZZ, Shen B, Zhao YL, Zhou Q, Yang Y, Li W, Yang PY, Yang YG
Nature communications 2023 Feb 16;14(1):863

DNMT1 determines osteosarcoma cell resistance to apoptosis by associatively modulating DNA and mRNA cytosine-5 methylation.

Shao D, Liu C, Wang Y, Lin J, Cheng X, Han P, Li Z, Jian D, Nie J, Jiang M, Wei Y, Xing J, Guo Z, Wang W, Yi X, Tang H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Dec;37(12):e23284

NSUN2-mediated m5C modification of HBV RNA positively regulates HBV replication.

Feng J, Xu T, He M, Li J, Yao P, Ma C, Yang S, Xu Z, Yan K, Chen X, Wang H, Liu J, Zeng C, Xia Y, Yan H, Zhou L, Chen Y
PLoS pathogens 2023 Dec;19(12):e1011808

NSUN2-mediated M(5)c methylation of IRF3 mRNA negatively regulates type I interferon responses during various viral infections.

Wang H, Feng J, Zeng C, Liu J, Fu Z, Wang D, Wang Y, Zhang L, Li J, Jiang A, He M, Cao Y, Yan K, Tang H, Guo D, Xu K, Zhou X, Zhou L, Lan K, Zhou Y, Chen Y
Emerging microbes & infections 2023 Dec;12(1):2178238

SRSF2 plays an unexpected role as reader of m(5)C on mRNA, linking epitranscriptomics to cancer.

Ma HL, Bizet M, Soares Da Costa C, Murisier F, de Bony EJ, Wang MK, Yoshimi A, Lin KT, Riching KM, Wang X, Beckman JI, Arya S, Droin N, Calonne E, Hassabi B, Zhang QY, Li A, Putmans P, Malbec L, Hubert C, Lan J, Mies F, Yang Y, Solary E, Daniels DL, Gupta YK, Deplus R, Abdel-Wahab O, Yang YG, Fuks F
Molecular cell 2023 Dec 7;83(23):4239-4254.e10

RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages.

Susanto TT, Hung V, Levine AG, Kerr CH, Yoo Y, Chen Y, Oses-Prieto JA, Fromm L, Fujii K, Wernig M, Burlingame AL, Ruggero D, Barna M
bioRxiv : the preprint server for biology 2023 Dec 7;

tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis.

Ying S, Li P, Wang J, Chen K, Zou Y, Dai M, Xu K, Feng G, Zhang C, Jiang H, Li W, Zhang Y, Zhou Q
Signal transduction and targeted therapy 2023 Apr 3;8(1):144

Light Emitting Diodes Irradiation Regulates miRNA-877-3p to Promote Cardiomyocyte Proliferation.

Gao X, Li H, Wang X, Ren Z, Tian Y, Zhao J, Qi W, Wang H, Yu Y, Gong R, Chen H, Ji H, Yang F, Ma W, Liu Y
International journal of medical sciences 2022;19(8):1254-1264

NSUN2 Promotes Tumor Progression and Regulates Immune Infiltration in Nasopharyngeal Carcinoma.

Tong X, Xiang Y, Hu Y, Hu Y, Li H, Wang H, Zhao KN, Xue X, Zhu S
Frontiers in oncology 2022;12:788801

Distinct Roles of m(5)C RNA Methyltransferase NSUN2 in Major Gynecologic Cancers.

Wang L, Zhang J, Su Y, Maimaitiyiming Y, Yang S, Shen Z, Lin S, Shen S, Zhan G, Wang F, Hsu CH, Cheng X
Frontiers in oncology 2022;12:786266

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity.

Li D, Yang J, Malik V, Huang Y, Huang X, Zhou H, Wang J
Nucleic acids research 2022 Nov 28;50(21):12462-12479

m(5) C and m(6) A modification of long noncoding NKILA accelerates cholangiocarcinoma progression via the miR-582-3p-YAP1 axis.

Zheng H, Zhu M, Li W, Zhou Z, Wan X
Liver international : official journal of the International Association for the Study of the Liver 2022 May;42(5):1144-1157

Developmental mRNA m(5)C landscape and regulatory innovations of massive m(5)C modification of maternal mRNAs in animals.

Liu J, Huang T, Chen W, Ding C, Zhao T, Zhao X, Cai B, Zhang Y, Li S, Zhang L, Xue M, He X, Ge W, Zhou C, Xu Y, Zhang R
Nature communications 2022 May 5;13(1):2484

Reactivity-dependent profiling of RNA 5-methylcytidine dioxygenases.

Arguello AE, Li A, Sun X, Eggert TW, Mairhofer E, Kleiner RE
Nature communications 2022 Jul 19;13(1):4176

NSUN2-mediated RNA m(5)C modification modulates uveal melanoma cell proliferation and migration.

Luo G, Xu W, Chen X, Wang S, Wang J, Dong F, Hu DN, Reinach PS, Yan D
Epigenetics 2022 Aug;17(8):922-933

Reorganization of the Landscape of Translated mRNAs in NSUN2-Deficient Cells and Specific Features of NSUN2 Target mRNAs.

Kossinova OA, Gopanenko AV, Babaylova ES, Tupikin AE, Kabilov MR, Malygin AA, Karpova GG
International journal of molecular sciences 2022 Aug 28;23(17)

Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling.

Zhai Y, Huang X, Zhang K, Huang Y, Jiang Y, Cui J, Zhang Z, Chiu CKC, Zhong W, Li G
Nature communications 2022 Aug 20;13(1):4906

NSUN2 modified by SUMO-2/3 promotes gastric cancer progression and regulates mRNA m5C methylation.

Hu Y, Chen C, Tong X, Chen S, Hu X, Pan B, Sun X, Chen Z, Shi X, Hu Y, Shen X, Xue X, Lu M
Cell death & disease 2021 Sep 9;12(9):842

Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC.

Ohshiro T, Konno M, Asai A, Komoto Y, Yamagata A, Doki Y, Eguchi H, Ofusa K, Taniguchi M, Ishii H
Scientific reports 2021 Sep 29;11(1):19304

Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.

Dai W, Li A, Yu NJ, Nguyen T, Leach RW, Wühr M, Kleiner RE
Nature chemical biology 2021 Nov;17(11):1178-1187

Novel insights into the role of 5-Methylcytosine RNA methylation in human abdominal aortic aneurysm.

He Y, Zhang H, Yin F, Guo P, Wang S, Wu Y, Xin S, Han Y, Zhang J
Frontiers in bioscience (Landmark edition) 2021 Nov 30;26(11):1147-1165

RNA methyltransferase NSUN2 promotes hypopharyngeal squamous cell carcinoma proliferation and migration by enhancing TEAD1 expression in an m(5)C-dependent manner.

Chen L, Ding J, Wang B, Chen X, Ying X, Yu Z, Dong P
Experimental cell research 2021 Jul 15;404(2):112664

Neuronal Nsun2 deficiency produces tRNA epitranscriptomic alterations and proteomic shifts impacting synaptic signaling and behavior.

Blaze J, Navickas A, Phillips HL, Heissel S, Plaza-Jennings A, Miglani S, Asgharian H, Foo M, Katanski CD, Watkins CP, Pennington ZT, Javidfar B, Espeso-Gil S, Rostandy B, Alwaseem H, Hahn CG, Molina H, Cai DJ, Pan T, Yao WD, Goodarzi H, Haghighi F, Akbarian S
Nature communications 2021 Aug 13;12(1):4913

Disease Activity-Associated Alteration of mRNA m(5) C Methylation in CD4(+) T Cells of Systemic Lupus Erythematosus.

Guo G, Wang H, Shi X, Ye L, Yan K, Chen Z, Zhang H, Jin Z, Xue X
Frontiers in cell and developmental biology 2020;8:430

CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.

Fang L, Wang W, Li G, Zhang L, Li J, Gan D, Yang J, Tang Y, Ding Z, Zhang M, Zhang W, Deng D, Song Z, Zhu Q, Cui H, Hu Y, Chen W
Molecular systems biology 2020 Nov;16(11):e10025

Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures.

Begik O, Lucas MC, Liu H, Ramirez JM, Mattick JS, Novoa EM
Genome biology 2020 May 7;21(1):97

Epitranscriptomic 5-Methylcytosine Profile in PM(2.5)-induced Mouse Pulmonary Fibrosis.

Han X, Liu H, Zhang Z, Yang W, Wu C, Liu X, Zhang F, Sun B, Zhao Y, Jiang G, Yang YG, Ding W
Genomics, proteomics & bioinformatics 2020 Feb;18(1):41-51

5-methylcytosine modification of an Epstein-Barr virus noncoding RNA decreases its stability.

Henry BA, Kanarek JP, Kotter A, Helm M, Lee N
RNA (New York, N.Y.) 2020 Aug;26(8):1038-1048

Multiple links between 5-methylcytosine content of mRNA and translation.

Schumann U, Zhang HN, Sibbritt T, Pan A, Horvath A, Gross S, Clark SJ, Yang L, Preiss T
BMC biology 2020 Apr 15;18(1):40

NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs.

Van Haute L, Lee SY, McCann BJ, Powell CA, Bansal D, Vasiliauskaitė L, Garone C, Shin S, Kim JS, Frye M, Gleeson JG, Miska EA, Rhee HW, Minczuk M
Nucleic acids research 2019 Sep 19;47(16):8720-8733

NOP2/Sun RNA methyltransferase 2 promotes tumor progression via its interacting partner RPL6 in gallbladder carcinoma.

Gao Y, Wang Z, Zhu Y, Zhu Q, Yang Y, Jin Y, Zhang F, Jiang L, Ye Y, Li H, Zhang Y, Liang H, Xiang S, Miao H, Liu Y, Hao Y
Cancer science 2019 Nov;110(11):3510-3519

Genome-wide identification of mRNA 5-methylcytosine in mammals.

Huang T, Chen W, Liu J, Gu N, Zhang R
Nature structural & molecular biology 2019 May;26(5):380-388

Cytosine-5 RNA methylation links protein synthesis to cell metabolism.

Gkatza NA, Castro C, Harvey RF, Heiß M, Popis MC, Blanco S, Bornelöv S, Sajini AA, Gleeson JG, Griffin JL, West JA, Kellner S, Willis AE, Dietmann S, Frye M
PLoS biology 2019 Jun;17(6):e3000297

Extensive Epitranscriptomic Methylation of A and C Residues on Murine Leukemia Virus Transcripts Enhances Viral Gene Expression.

Courtney DG, Chalem A, Bogerd HP, Law BA, Kennedy EM, Holley CL, Cullen BR
mBio 2019 Jun 11;10(3)

5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.

Chen X, Li A, Sun BF, Yang Y, Han YN, Yuan X, Chen RX, Wei WS, Liu Y, Gao CC, Chen YS, Zhang M, Ma XD, Liu ZW, Luo JH, Lyu C, Wang HL, Ma J, Zhao YL, Zhou FJ, Huang Y, Xie D, Yang YG
Nature cell biology 2019 Aug;21(8):978-990

Epitranscriptomic Addition of m(5)C to HIV-1 Transcripts Regulates Viral Gene Expression.

Courtney DG, Tsai K, Bogerd HP, Kennedy EM, Law BA, Emery A, Swanstrom R, Holley CL, Cullen BR
Cell host & microbe 2019 Aug 14;26(2):217-227.e6

5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m(5)C reader.

Yang X, Yang Y, Sun BF, Chen YS, Xu JW, Lai WY, Li A, Wang X, Bhattarai DP, Xiao W, Sun HY, Zhu Q, Ma HL, Adhikari S, Sun M, Hao YJ, Zhang B, Huang CM, Huang N, Jiang GB, Zhao YL, Wang HL, Sun YP, Yang YG
Cell research 2017 May;27(5):606-625

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Simsek D, Tiu GC, Flynn RA, Byeon GW, Leppek K, Xu AF, Chang HY, Barna M
Cell 2017 Jun 1;169(6):1051-1065.e18

Compound Heterozygosity for Y Box Proteins Causes Sterility Due to Loss of Translational Repression.

Snyder E, Soundararajan R, Sharma M, Dearth A, Smith B, Braun RE
PLoS genetics 2015 Dec;11(12):e1005690

tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells.

Okamoto M, Fujiwara M, Hori M, Okada K, Yazama F, Konishi H, Xiao Y, Qi G, Shimamoto F, Ota T, Temme A, Tatsuka M
PLoS genetics 2014 Sep;10(9):e1004639

Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.

Khoddami V, Cairns BR
Nature protocols 2014 Feb;9(2):337-61

Identification of direct targets and modified bases of RNA cytosine methyltransferases.

Khoddami V, Cairns BR
Nature biotechnology 2013 May;31(5):458-64

The mouse cytosine-5 RNA methyltransferase NSun2 is a component of the chromatoid body and required for testis differentiation.

Hussain S, Tuorto F, Menon S, Blanco S, Cox C, Flores JV, Watt S, Kudo NR, Lyko F, Frye M
Molecular and cellular biology 2013 Apr;33(8):1561-70

Whole exome sequencing identifies a splicing mutation in NSUN2 as a cause of a Dubowitz-like syndrome.

Martinez FJ, Lee JH, Lee JE, Blanco S, Nickerson E, Gabriel S, Frye M, Al-Gazali L, Gleeson JG
Journal of medical genetics 2012 Jun;49(6):380-5

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20854-1-AP(NSUN2 antibody) at dilution of 1:800
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using NSUN2 antibody 20854-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 20854-1-AP(NSUN2 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 20854-1-AP(NSUN2 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue