20854-1-AP

antibody from Proteintech Group

Targeting: NSUN2 FLJ20303, Misu, MRT5, TRM4

Provider product page for 20854-1-AP

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Antibody data

Antibody Data
Antigen structure
References [112]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 20854-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20854-1-AP, RRID:AB_10693629
Product name: NSUN2 antibody
Antibody type: Polyclonal
Description: KD/KO validated NSUN2 antibody (Cat. #20854-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NSUN2 protein structure - 20854-1-AP shown in red.

Submitted references Targeting the NSUN2-DHODH axis reverses ferroptosis resistance and oxaliplatin resistance in colorectal cancer.

Zhang J, Shen J, Zeng T, Gao C, Sheng B, Li J, Zeng W, Shen J, Shen C, Wang J, Wang J
Frontiers in pharmacology 2026;17:1739981

Lactylation-driven NSUN2-mediated RNA m5C modification promotes perineural invasion in pancreatic cancer.

Huang T, Hu C, Chen H, Jiang H, Li T, Tian Q, He R, Yuan Y, Jiang Y, Zhou Y, Lin Q, Li Z, Xiao M, Wei X, Chen R, Zheng S
Theranostics 2026;16(4):1782-1803

Faecalibacterium prausnitzii: A microbial ally against preeclampsia via extracellular vesicle-mediated ferroptosis inhibition.

Zhang W, Lai W, Peng M, Yu L, Nie Y, Deng Y
Free radical biology & medicine 2026 May;248:333-349

The oncogenic role of NSUN2 in lung adenocarcinoma by stabilizing CCT5 mRNA via a YBX1-dependent m5C modification.

Li M, Du N, Tang P
Molecular and cellular biochemistry 2026 Jan;481(1):231-243

Oncogenic and immunological roles of NSUN2 in triple negative breast cancer.

An N, Zeng YF, Tian ZW, Tian J, Xu LF, Yang YL, Zheng HM, Zhang JW, Xi YQ
Scientific reports 2026 Jan 6;16(1):894

NCAPG confers ferroptosis resistance of hepatocellular carcinoma through NSUN2-mediated m5C modification.

Huang Z, Wang J, He A, Feng Q, Yang J, Wu L, Liao W
Cellular signalling 2026 Feb;138:112256

LncRNA SLC16A1-AS1 cooperates with NSUN2 to stabilize GRP78 mRNA via m5C modification in gastric cancer.

Zheng B, Zhao W, Jin Y, Sun W, Li Y, Zhang Q, Li Y, Wang J, Chen D, Ren K, Zhang W, Chen Y, Yuan L, Wang XJ, Yang F, Chen J
iScience 2026 Feb 20;29(2):114614

DUX4-induced HSATII RNA accumulation drives protein aggregation, impacting RNA processing pathways.

Arends T, Bennett SR, Tapscott SJ
The Journal of cell biology 2026 Feb 2;225(2)

Coronaviruses reprogram the tRNA epitranscriptome to favor viral protein expression.

Muscolino E, Puig-Torrents M, Buigues Bisquert J, Correa Mendonca D, Talló-Parra M, Perez-Vilaro G, Caño-Prades O, Meehan GR, Kerr K, Herder V, Chillón M, Castello A, Sanjuan R, Patel AH, Díez J
Nature communications 2026 Feb 19;17(1)

M(5)C-driven stabilization of SERPINB5 promotes cervical cancer progression and chemotherapy resistance.

Liu J, Zhou L, Yao P, Zhang N, Guo X, Chen F, Yang S, Du X, Wang H, Zhou Y, Chen Y, Zhou L
Cell death & disease 2026 Feb 11;17(1):215

NSUN2 restrains renal fibrosis via m5C-stabilized GPX4 and ferroptosis inhibition.

Zan Y, Chen L, Ma L, Meng P, Ma S, Liu H, Tian Y, He X, Wang Z, Ding Y
Cellular signalling 2026 Aug;144:112543

Unveiling the potential impact of RNA m5C methyltransferases NSUN2 and NSUN6 on cellular aging.

Zhou J, Han Y, Ji F, Zhang R, Liang Y, Zhao X, Hou R
Frontiers in genetics 2025;16:1477542

NSUN2 promotes PM(2.5)-induced epithelial-mesenchymal transition through methylating chitinase 3-like-1 mRNA.

Shao M, Li C, Li J, Chen R, Wang Y, Su J, Tu Y, Zhang F, Zhang X, Ding W
Journal of hazardous materials 2025 Sep 5;495:138883

Nanopuerarin Improves Doxorubicin Therapeutic Efficacy by Inhibiting Angiogenesis via Regulating NSUN2 in Hepatocellular Carcinoma.

Feng N, Chang J, Yu H, Li T, Lei L, Huang X, Shi D, Liu R, Zhang W, He X
ACS omega 2025 Sep 2;10(34):38452-38462

NSUN2-mediated m5C hypermethylation of hsa_circ_0004516 promotes breast cancer brain metastasis by activating AKT signaling.

Li M, Li J, Wen H, Li J, Wang S, Guo J, Zhang D, Zhang A, Cui C, Fu R, An M, Zhang W, Fu B
Non-coding RNA research 2025 Oct;14:177-190

mRNA turnover dynamics are affected by cell differentiation and loss of the cytosine methyltransferase Nsun2.

Delazer I, Bauer I, Rummel T, Nykiel K, Rieder D, Fickl M, Tumler V, Razkova A, Schaefer MR, Scheed T, Erlacher MD, Erhard F, Micura R, Lusser A
Nucleic acids research 2025 Oct 14;53(19)

Dynamic landscape and regulation of m(5)C methylation in human tissues.

An K, Liu R, Yang H, Feng L, Zhang X, Zhang X, Hou M, Wei J, Han X, Shi B, Yao H, Chen X, Wang H, Tian X
Science China. Life sciences 2025 Nov;68(11):3203-3216

FOXA1-dependent NSUN2 facilitates the advancement of prostate cancer by preserving TRIM28 mRNA stability in a m5C-dependent manner.

Wang Z, Mierxiati A, Zhu W, Li T, Xu H, Wan F, Ye D
NPJ precision oncology 2025 May 3;9(1):127

NSUN2-mediated HCV RNA m5C Methylation Facilitates Viral RNA Stability and Replication.

Li ZL, Xie Y, Wang Y, Wang J, Zhou X, Zhang XL
Genomics, proteomics & bioinformatics 2025 May 10;23(1)

Targeting YBX1-m5C mediates RNF115 mRNA circularisation and translation to enhance vulnerability of ferroptosis in hepatocellular carcinoma.

Li O, An K, Wang H, Li X, Wang Y, Huang L, Du Y, Qin N, Dong J, Wei J, Sun R, Shi Y, Guo Y, Sun X, Yang Y, Yang YG, Kan Q, Tian X
Clinical and translational medicine 2025 Mar;15(3):e70270

piRNA28846 has the potential to be a novel RNA nucleic acid drug for ovarian cancer.

Qin HL, Han Y, Li JQ, Wu QH, Du YP, Li QH, Chen X, Wang YP, Guan X, Sheng XJ, Chen S, Zhao Y
NPJ precision oncology 2025 Mar 8;9(1):65

NONO regulates m(5)C modification and alternative splicing of PTEN mRNAs to drive gastric cancer progression.

Zhao G, Liu R, Ge L, Qi D, Wu Q, Lin Z, Song H, Zhong L, Cui H
Journal of experimental & clinical cancer research : CR 2025 Mar 4;44(1):81

NSUN2/ALYREF axis-driven m(5)C methylation enhances PD-L1 expression and facilitates immune evasion in non-small-cell lung cancer.

Yang Y, Cao L, Xu X, Li D, Deng Y, Li L, Zeng B, Jiang H, Shan L, Huang Y, Xu Y, Ma L
Cancer immunology, immunotherapy : CII 2025 Mar 3;74(4):132

YBX1 promotes 5-Fluorouracil resistance in gastric cancer via m5C-dependent ATG9A mRNA stabilization through autophagy.

Huang H, Fang L, Zhu C, Lv J, Xu P, Chen Z, Zhang Z, Wang J, Wang W, Xu Z
Oncogene 2025 Jul;44(28):2357-2371

NSUN2-mediated RNA m(5)C modification drives multiple myeloma progression by enhancing the stability of HIP1 mRNA.

Jiang Y, Sun J, Chen Y, Cheng L, Feng S, Wang Y, Sun C
Scientific reports 2025 Jul 31;15(1):27888

NSUN2-mediated cytosine-5 methylation of FSP1 protects acute myeloid leukemia cells from ferroptosis.

Ye W, Zhao Y, Zhou Y, Huang J, He X, Ma Z, Huang X, Hu C, Li F, Ling Q, Wang H, Tong H, Sun J, Jin J
Molecular cancer 2025 Jul 21;24(1):201

p38α-eIF6-Nsun2 axis promotes ILC3's rapid response to protect host from intestinal inflammation.

Huang J, Zhang J, Song P, Huang J, Yang Z, Han J, Wu L, Guo X
The Journal of experimental medicine 2025 Jan 6;222(1)

DUX4-induced HSATII RNA accumulation drives protein aggregation impacting RNA processing pathways.

Arends T, Bennett SR, Tapscott SJ
bioRxiv : the preprint server for biology 2025 Jan 22;

NSUN2 lactylation drives cancer cell resistance to ferroptosis through enhancing GCLC-dependent glutathione synthesis.

Niu K, Chen Z, Li M, Ma G, Deng Y, Zhang J, Wei D, Wang J, Zhao Y
Redox biology 2025 Feb;79:103479

Manganese Galvanic Cells Intervene in Tumor Metabolism to Reinforce cGAS-STING Activation for Bidirectional Synergistic Hydrogen-Immunotherapy.

Yang N, Sun S, Xu J, Gong F, Lei H, Hao Y, Pei Z, Wang C, Yu Q, Nie J, Jiang N, Ni C, Cheng L
Advanced materials (Deerfield Beach, Fla.) 2025 Feb;37(8):e2414929

Novel m(5)C-dependent post-transcriptional regulation of KIF11 drives lymphatic metastasis in gastric cancer.

Zhang Y, He R, Ye J, Xu X, Gui X, Wu Y, Lin X, Lin X, Chen K
Scientific reports 2025 Dec 29;15(1):44782

NSUN2 mediated-aberrant 5-methylcytosine methylation regulates autophagy-related ferroptosis in oral squamous cell carcinoma progression.

Lu Y, Li R, Du W, Wu J, He Y, Yuan L, Chen X, Lv S, Shi F, Hu J, Zhao W, Yu D
Cell death & disease 2025 Dec 23;16(1):903

NSUN2 Negatively Regulates TP53 mRNA Stability to Promote the Malignant Progression of Nasopharyngeal Carcinoma.

Zheng L, Wei J, Li X, Li M, Xue C, Chen S, Wei Q, Fan S, Xiong W, Zhou M, Deng H
Cancers 2025 Dec 10;17(24)

Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing.

Zhou J, Zhao D, Li J, Kong D, Li X, Zhang R, Liang Y, Gao X, Qian Y, Wang D, Chen J, Lai L, Han Y, Li Z
eLife 2025 Apr 8;13

LINC02167 stabilizes KSR1 mRNA in an m(5)C-dependent manner to regulate the ERK/MAPK signaling pathway and promotes colorectal cancer metastasis.

Qi J, Jiang T, Liu B, Hu Q, Chen J, Ma N, Xu Y, Song H, Song J
Journal of experimental & clinical cancer research : CR 2025 Apr 15;44(1):121

Substrate diversity of NSUN enzymes and links of 5-methylcytosine to mRNA translation and turnover.

Guarnacci M, Zhang PH, Kanchi M, Hung YT, Lin H, Shirokikh NE, Yang L, Preiss T
Life science alliance 2024 Sep;7(9)

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages.

Susanto TT, Hung V, Levine AG, Chen Y, Kerr CH, Yoo Y, Oses-Prieto JA, Fromm L, Zhang Z, Lantz TC, Fujii K, Wernig M, Burlingame AL, Ruggero D, Barna M
Molecular cell 2024 Sep 19;84(18):3545-3563.e25

Vitamin D3 regulates NSUN2 expression and inhibits melanoma cell proliferation and migration.

Wang L, Zhang Q, Wang J, Lu H, Zeng W, Zhang T
Molecular diversity 2024 Oct;28(5):2863-2874

Microbiome-induced reprogramming in post-transcriptional landscape using nanopore direct RNA sequencing.

Xu Z, Zheng X, Fan J, Jiao Y, Huang S, Xie Y, Xu S, Lu Y, Liu A, Liu R, Yang Y, Luo GZ, Pan T, Wang X
Cell reports 2024 Oct 22;43(10):114798

NSUN2 regulates Wnt signaling pathway depending on the m5C RNA modification to promote the progression of hepatocellular carcinoma.

Xing H, Gu X, Liu Y, Xu L, He Y, Xue C
Oncogene 2024 Nov;43(47):3469-3482

NSUN2 Promotes Head and Neck Squamous Cell Carcinoma Progression by Targeting EMT-Related Gene LAMC2 in an m(5)C-YBX1-Dependent Manner.

Huang S, Cao C, Tang D, Liu Y, Zhou W, Liu L, Zheng X, He Q, Wang A
Biomedicines 2024 Nov 6;12(11)

LncRNA HOST2 promotes NSUN2-mediated breast cancer progression via interaction with ELAVL1.

Wang X, Liu D, Hua K, Fang L
Cellular signalling 2024 May;117:111112

Prediction of m6A and m5C at single-molecule resolution reveals a transcriptome-wide co-occurrence of RNA modifications.

Acera Mateos P, J Sethi A, Ravindran A, Srivastava A, Woodward K, Mahmud S, Kanchi M, Guarnacci M, Xu J, W S Yuen Z, Zhou Y, Sneddon A, Hamilton W, Gao J, M Starrs L, Hayashi R, Wickramasinghe V, Zarnack K, Preiss T, Burgio G, Dehorter N, E Shirokikh N, Eyras E
Nature communications 2024 May 9;15(1):3899

NSUN2 affects diabetic retinopathy progression by regulating MUC1 expression through RNA m(5)C methylation.

Wang R, Xue W, Kan F, Zhang H, Wang D, Wang L, Wang J
Journal of translational medicine 2024 May 19;22(1):476

RNA m(5)C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer.

Liu X, Wei Q, Yang C, Zhao H, Xu J, Mobet Y, Luo Q, Yang D, Zuo X, Chen N, Yang Y, Li L, Wang W, Yu J, Xu J, Liu T, Yi P
Experimental & molecular medicine 2024 Mar;56(3):600-615

NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA stabilization.

Zou S, Huang Y, Yang Z, Zhang J, Meng M, Zhang Y, Feng J, Sun R, Li W, Wang W, López JG, Fang L
Clinical and translational medicine 2024 Mar;14(3):e1621

Changes of RNA m(6)A/m(5)C Modification Regulatory Molecules in Ferroptosis of T2DM Rat Pancreas.

Liu X, Wang N, Gu S, He Z
Cell biochemistry and biophysics 2024 Jun;82(2):1279-1289

Suppression of NSUN2 enhances the sensitivity to chemosensitivity and inhibits proliferation by mediating cell apoptosis in gastric cancer.

Shen X, Sun H, Shu S, Tang W, Yuan Y, Su H, Li Y, Fan H
Pathology, research and practice 2024 Jan;253:154986

Epigenetic addition of m(5)C to HBV transcripts promotes viral replication and evasion of innate antiviral responses.

Ding S, Liu H, Liu L, Ma L, Chen Z, Zhu M, Liu L, Zhang X, Hao H, Zuo L, Yang J, Wu X, Zhou P, Huang F, Zhu F, Guan W
Cell death & disease 2024 Jan 12;15(1):39

m5C modification of LINC00324 promotes angiogenesis in glioma through CBX3/VEGFR2 pathway.

Pan A, Xue Y, Ruan X, Dong W, Wang D, Liu Y, Liu L, Lin Y, E T, Lin H, Xu H, Liu X, Wang P
International journal of biological macromolecules 2024 Feb;257(Pt 1):128409

ALYREF-JunD-SLC7A5 axis promotes pancreatic ductal adenocarcinoma progression through epitranscriptome-metabolism reprogramming and immune evasion.

Meng Q, Xie Y, Sun K, He L, Wu H, Zhang Q, Liang T
Cell death discovery 2024 Feb 24;10(1):97

Integrative Analysis Identifies NSUN2 as an Essential Coordinator for Glioma Malignancy and Glucose Metabolism.

He Y, Yuan Y, Ji L, Shu Y, Wang Z, Zhang S, Yang W, Chen M, Liu Y
The journal of gene medicine 2024 Dec;26(12):e70004

Glucose binds and activates NSUN2 to promote translation and epidermal differentiation.

Miao W, Porter DF, Li Y, Meservey LM, Yang YY, Ma C, Ferguson ID, Tien VB, Jack TM, Ducoli L, Lopez-Pajares V, Tao S, Savage PB, Wang Y, Khavari PA
Nucleic acids research 2024 Dec 11;52(22):13577-13593

NSUN2 is critical for mouse oocyte meiosis and early embryonic development.

Xu T, Gao M, Zhang L, Cao T, Qiu Y, Liu S, Wu W, Zhou Y, Liu H, Zhang R, Ruan X, Huang J
Reproduction (Cambridge, England) 2024 Dec 1;168(6)

Epitranscriptomic m(5)C methylation of SARS-CoV-2 RNA regulates viral replication and the virulence of progeny viruses in the new infection.

Wang H, Feng J, Fu Z, Xu T, Liu J, Yang S, Li Y, Deng J, Zhang Y, Guo M, Wang X, Zhang Z, Huang Z, Lan K, Zhou L, Chen Y
Science advances 2024 Aug 9;10(32):eadn9519

Base-resolution m(5)C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach.

Lu L, Zhang X, Zhou Y, Shi Z, Xie X, Zhang X, Gao L, Fu A, Liu C, He B, Xiong X, Yin Y, Wang Q, Yi C, Li X
Molecular cell 2024 Aug 8;84(15):2984-3000.e8

ALYREF promotes the metastasis of nasopharyngeal carcinoma by increasing the stability of NOTCH1 mRNA.

Jin Y, Yao J, Fu J, Huang Q, Luo Y, You Y, Zhang W, Zhong Q, Xia T, Xia L
Cell death & disease 2024 Aug 8;15(8):578

5-Methylcytosine transferase NSUN2 drives NRF2-mediated ferroptosis resistance in non-small cell lung cancer.

Chen Y, Jiang Z, Zhang C, Zhang L, Chen H, Xiao N, Bai L, Liu H, Wan J
The Journal of biological chemistry 2024 Apr;300(4):106793

NSUN2 relies on ALYREF to regulate Nrf2-mediated oxidative stress and alleviate Dox-induced liver injury.

Huang Y, Li X, Wei L, Ma S, Ma L, Zan Y, He X, Tang Y, Ding Y
Biology direct 2024 Apr 29;19(1):32

Upregulation of LRRC8A by m(5)C modification-mediated mRNA stability suppresses apoptosis and facilitates tumorigenesis in cervical cancer.

Chen Y, Zuo X, Wei Q, Xu J, Liu X, Liu S, Wang H, Luo Q, Wang Y, Yang Y, Zhao H, Xu J, Liu T, Yi P
International journal of biological sciences 2023;19(2):691-704

NOP2/Sun RNA methyltransferase 2 is a potential pan-cancer prognostic biomarker and is related to immunity.

Zheng L, Li M, Wei J, Chen S, Xue C, Duan Y, Tang F, Li G, Xiong W, She K, Deng H, Zhou M
PloS one 2023;18(9):e0292212

RNA cytosine methyltransferase NSUN5 promotes protein synthesis and tumorigenic phenotypes in glioblastoma.

Zhou J, Kong YS, Vincent KM, Dieters-Castator D, Bukhari AB, Glubrecht D, Liu RZ, Quilty D, Findlay SD, Huang X, Xu Z, Yang RZ, Zhang L, Tang E, Lajoie G, Eisenstat DD, Gamper AM, Fahlman R, Godbout R, Postovit LM, Fu Y
Molecular oncology 2023 Sep;17(9):1763-1783

RNA 5-Methylcytosine regulators are associated with cell adhesion and predict prognosis of endometrial cancer.

Yang S, Luo Y, Zhou D, Xiang J, Xi X
Translational cancer research 2023 Oct 31;12(10):2556-2571

NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance.

Chen T, Xu ZG, Luo J, Manne RK, Wang Z, Hsu CC, Pan BS, Cai Z, Tsai PJ, Tsai YS, Chen ZZ, Li HY, Lin HK
Cell metabolism 2023 Oct 3;35(10):1782-1798.e8

NSUN2-mediated m(5) C RNA methylation dictates retinoblastoma progression through promoting PFAS mRNA stability and expression.

Zuo S, Li L, Wen X, Gu X, Zhuang A, Li R, Ye F, Ge S, Fan X, Fan J, Chai P, Lu L
Clinical and translational medicine 2023 May;13(5):e1273

RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation.

Kim YA, Siddiqui T, Blaze J, Cosacak MI, Winters T, Kumar A, Tein E, Sproul AA, Teich AF, Bartolini F, Akbarian S, Kizil C, Hargus G, Santa-Maria I
Acta neuropathologica 2023 Jan;145(1):29-48

NSUN2 alleviates doxorubicin-induced myocardial injury through Nrf2-mediated antioxidant stress.

Wang Y, Zan Y, Huang Y, Peng X, Ma S, Ren J, Li X, Wei L, Wang X, Yuan Y, Tang J, Zhan Z, Wang Z, Ding Y
Cell death discovery 2023 Feb 4;9(1):43

m(5)C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization.

Wang N, Chen RX, Deng MH, Wei WS, Zhou ZH, Ning K, Li YH, Li XD, Ye YL, Wen JH, Dong B, Zhang XP, Liu ZW, Zhou FJ
Cell death & disease 2023 Feb 18;14(2):139

Nsun2 coupling with RoRγt shapes the fate of Th17 cells and promotes colitis.

Yang WL, Qiu W, Zhang T, Xu K, Gu ZJ, Zhou Y, Xu HJ, Yang ZZ, Shen B, Zhao YL, Zhou Q, Yang Y, Li W, Yang PY, Yang YG
Nature communications 2023 Feb 16;14(1):863

DNMT1 determines osteosarcoma cell resistance to apoptosis by associatively modulating DNA and mRNA cytosine-5 methylation.

Shao D, Liu C, Wang Y, Lin J, Cheng X, Han P, Li Z, Jian D, Nie J, Jiang M, Wei Y, Xing J, Guo Z, Wang W, Yi X, Tang H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Dec;37(12):e23284

NSUN2-mediated m5C modification of HBV RNA positively regulates HBV replication.

Feng J, Xu T, He M, Li J, Yao P, Ma C, Yang S, Xu Z, Yan K, Chen X, Wang H, Liu J, Zeng C, Xia Y, Yan H, Zhou L, Chen Y
PLoS pathogens 2023 Dec;19(12):e1011808

NSUN2-mediated M(5)c methylation of IRF3 mRNA negatively regulates type I interferon responses during various viral infections.

Wang H, Feng J, Zeng C, Liu J, Fu Z, Wang D, Wang Y, Zhang L, Li J, Jiang A, He M, Cao Y, Yan K, Tang H, Guo D, Xu K, Zhou X, Zhou L, Lan K, Zhou Y, Chen Y
Emerging microbes & infections 2023 Dec;12(1):2178238

SRSF2 plays an unexpected role as reader of m(5)C on mRNA, linking epitranscriptomics to cancer.

Ma HL, Bizet M, Soares Da Costa C, Murisier F, de Bony EJ, Wang MK, Yoshimi A, Lin KT, Riching KM, Wang X, Beckman JI, Arya S, Droin N, Calonne E, Hassabi B, Zhang QY, Li A, Putmans P, Malbec L, Hubert C, Lan J, Mies F, Yang Y, Solary E, Daniels DL, Gupta YK, Deplus R, Abdel-Wahab O, Yang YG, Fuks F
Molecular cell 2023 Dec 7;83(23):4239-4254.e10

RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages.

Susanto TT, Hung V, Levine AG, Kerr CH, Yoo Y, Chen Y, Oses-Prieto JA, Fromm L, Fujii K, Wernig M, Burlingame AL, Ruggero D, Barna M
bioRxiv : the preprint server for biology 2023 Dec 7;

tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis.

Ying S, Li P, Wang J, Chen K, Zou Y, Dai M, Xu K, Feng G, Zhang C, Jiang H, Li W, Zhang Y, Zhou Q
Signal transduction and targeted therapy 2023 Apr 3;8(1):144

Light Emitting Diodes Irradiation Regulates miRNA-877-3p to Promote Cardiomyocyte Proliferation.

Gao X, Li H, Wang X, Ren Z, Tian Y, Zhao J, Qi W, Wang H, Yu Y, Gong R, Chen H, Ji H, Yang F, Ma W, Liu Y
International journal of medical sciences 2022;19(8):1254-1264

NSUN2 Promotes Tumor Progression and Regulates Immune Infiltration in Nasopharyngeal Carcinoma.

Tong X, Xiang Y, Hu Y, Hu Y, Li H, Wang H, Zhao KN, Xue X, Zhu S
Frontiers in oncology 2022;12:788801

Distinct Roles of m(5)C RNA Methyltransferase NSUN2 in Major Gynecologic Cancers.

Wang L, Zhang J, Su Y, Maimaitiyiming Y, Yang S, Shen Z, Lin S, Shen S, Zhan G, Wang F, Hsu CH, Cheng X
Frontiers in oncology 2022;12:786266

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity.

Li D, Yang J, Malik V, Huang Y, Huang X, Zhou H, Wang J
Nucleic acids research 2022 Nov 28;50(21):12462-12479

m(5) C and m(6) A modification of long noncoding NKILA accelerates cholangiocarcinoma progression via the miR-582-3p-YAP1 axis.

Zheng H, Zhu M, Li W, Zhou Z, Wan X
Liver international : official journal of the International Association for the Study of the Liver 2022 May;42(5):1144-1157

Developmental mRNA m(5)C landscape and regulatory innovations of massive m(5)C modification of maternal mRNAs in animals.

Liu J, Huang T, Chen W, Ding C, Zhao T, Zhao X, Cai B, Zhang Y, Li S, Zhang L, Xue M, He X, Ge W, Zhou C, Xu Y, Zhang R
Nature communications 2022 May 5;13(1):2484

Reactivity-dependent profiling of RNA 5-methylcytidine dioxygenases.

Arguello AE, Li A, Sun X, Eggert TW, Mairhofer E, Kleiner RE
Nature communications 2022 Jul 19;13(1):4176

NSUN2-mediated RNA m(5)C modification modulates uveal melanoma cell proliferation and migration.

Luo G, Xu W, Chen X, Wang S, Wang J, Dong F, Hu DN, Reinach PS, Yan D
Epigenetics 2022 Aug;17(8):922-933

Reorganization of the Landscape of Translated mRNAs in NSUN2-Deficient Cells and Specific Features of NSUN2 Target mRNAs.

Kossinova OA, Gopanenko AV, Babaylova ES, Tupikin AE, Kabilov MR, Malygin AA, Karpova GG
International journal of molecular sciences 2022 Aug 28;23(17)

Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling.

Zhai Y, Huang X, Zhang K, Huang Y, Jiang Y, Cui J, Zhang Z, Chiu CKC, Zhong W, Li G
Nature communications 2022 Aug 20;13(1):4906

NSUN2 modified by SUMO-2/3 promotes gastric cancer progression and regulates mRNA m5C methylation.

Hu Y, Chen C, Tong X, Chen S, Hu X, Pan B, Sun X, Chen Z, Shi X, Hu Y, Shen X, Xue X, Lu M
Cell death & disease 2021 Sep 9;12(9):842

Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC.

Ohshiro T, Konno M, Asai A, Komoto Y, Yamagata A, Doki Y, Eguchi H, Ofusa K, Taniguchi M, Ishii H
Scientific reports 2021 Sep 29;11(1):19304

Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.

Dai W, Li A, Yu NJ, Nguyen T, Leach RW, Wühr M, Kleiner RE
Nature chemical biology 2021 Nov;17(11):1178-1187

Novel insights into the role of 5-Methylcytosine RNA methylation in human abdominal aortic aneurysm.

He Y, Zhang H, Yin F, Guo P, Wang S, Wu Y, Xin S, Han Y, Zhang J
Frontiers in bioscience (Landmark edition) 2021 Nov 30;26(11):1147-1165

RNA methyltransferase NSUN2 promotes hypopharyngeal squamous cell carcinoma proliferation and migration by enhancing TEAD1 expression in an m(5)C-dependent manner.

Chen L, Ding J, Wang B, Chen X, Ying X, Yu Z, Dong P
Experimental cell research 2021 Jul 15;404(2):112664

Neuronal Nsun2 deficiency produces tRNA epitranscriptomic alterations and proteomic shifts impacting synaptic signaling and behavior.

Blaze J, Navickas A, Phillips HL, Heissel S, Plaza-Jennings A, Miglani S, Asgharian H, Foo M, Katanski CD, Watkins CP, Pennington ZT, Javidfar B, Espeso-Gil S, Rostandy B, Alwaseem H, Hahn CG, Molina H, Cai DJ, Pan T, Yao WD, Goodarzi H, Haghighi F, Akbarian S
Nature communications 2021 Aug 13;12(1):4913

Disease Activity-Associated Alteration of mRNA m(5) C Methylation in CD4(+) T Cells of Systemic Lupus Erythematosus.

Guo G, Wang H, Shi X, Ye L, Yan K, Chen Z, Zhang H, Jin Z, Xue X
Frontiers in cell and developmental biology 2020;8:430

CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.

Fang L, Wang W, Li G, Zhang L, Li J, Gan D, Yang J, Tang Y, Ding Z, Zhang M, Zhang W, Deng D, Song Z, Zhu Q, Cui H, Hu Y, Chen W
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Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures.

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Epitranscriptomic 5-Methylcytosine Profile in PM(2.5)-induced Mouse Pulmonary Fibrosis.

Han X, Liu H, Zhang Z, Yang W, Wu C, Liu X, Zhang F, Sun B, Zhao Y, Jiang G, Yang YG, Ding W
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Extensive Epitranscriptomic Methylation of A and C Residues on Murine Leukemia Virus Transcripts Enhances Viral Gene Expression.

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20854-1-AP(NSUN2 antibody) at dilution of 1:800
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using NSUN2 antibody 20854-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 20854-1-AP(NSUN2 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 20854-1-AP(NSUN2 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue