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- Product number
- HPA037896 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA037896, RRID:AB_10670532
- Product name
- Anti-NSUN2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NSUN2, Gene description: NOP2/Sun RNA methyltransferase family, member 2, Alternative Gene Names: FLJ20303, Misu, MRT5, TRM4, Validated applications: IHC, WB, Uniprot ID: Q08J23, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.3 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references NSUN2/YBX1 promotes the progression of breast cancer by enhancing HGH1 mRNA stability through m5C methylation
Aberrant m5C hypermethylation mediates intrinsic resistance to gefitinib through NSUN2/YBX1/QSOX1 axis in EGFR-mutant non-small-cell lung cancer
Mammalian NSUN2 introduces 5-methylcytidines into mitochondrial tRNAs
Zhang X, An K, Ge X, Sun Y, Wei J, Ren W, Wang H, Wang Y, Du Y, He L, Li O, Zhou S, Shi Y, Ren T, Yang Y, Kan Q, Tian X
Breast Cancer Research 2024;26(1)
Breast Cancer Research 2024;26(1)
Aberrant m5C hypermethylation mediates intrinsic resistance to gefitinib through NSUN2/YBX1/QSOX1 axis in EGFR-mutant non-small-cell lung cancer
Wang Y, Wei J, Feng L, Li O, Huang L, Zhou S, Xu Y, An K, Zhang Y, Chen R, He L, Wang Q, Wang H, Du Y, Liu R, Huang C, Zhang X, Yang Y, Kan Q, Tian X
Molecular Cancer 2023;22(1)
Molecular Cancer 2023;22(1)
Mammalian NSUN2 introduces 5-methylcytidines into mitochondrial tRNAs
Shinoda S, Kitagawa S, Nakagawa S, Wei F, Tomizawa K, Araki K, Araki M, Suzuki T, Suzuki T
Nucleic Acids Research 2019;47(16):8734-8745
Nucleic Acids Research 2019;47(16):8734-8745
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Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting NSUN2 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting NSUN2 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:382
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NSUN2 antibody. Remaining relative intensity is presented.
- Sample type
- Human
- Protocol
- Protocol
