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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- MA5-56501 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSUN2 Recombinant Rabbit Monoclonal Antibody (PSH05-05)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- PSH05-05
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of NSUN2 with HeLa cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with NSUN2 Recombinant Monoclonal Antibody (Product # MA5-56501)at a dilution of 1:250 in 1% BSA in PBST overnight at 4 ℃. Followed by Goat Anti-Rabbit IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NSUN2 was immunoprecipitated from 0.2 mg HeLa cell lysate with NSUN2 Recombinant Monoclonal Antibody (Product # MA5-56501) at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using NSUN2 Recombinant Monoclonal Antibody (Product # MA5-56501) at 1:1,000 dilution. Followed by Anti-Rabbit IgG for IP Nano-secondary antibody at 1:5,000 dilution (1 hr, room temperature). Lane 1: HeLa cell lysate; Lane 2: (MA5-56501) IP in HeLa cell lysate; Lane 3: Rabbit IgG in HeLa cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 24 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NSUN2 with paraffin-embedded human testis tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with NSUN2 Recombinant Monoclonal Antibody (Product # MA5-56501) at a dilution of 1:1,000 (1 hr, room temp).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NSUN2 with paraffin-embedded human small intestine tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with NSUN2 Recombinant Monoclonal Antibody (Product # MA5-56501) at a dilution of 1:1,000 (1 hr, room temp).
