A305-805A-T

antibody from Invitrogen Antibodies

Targeting: XPO7 KIAA0745, RANBP16

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-805A-T

Provider

Invitrogen Antibodies

Product name

RanBP16 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RanBP16 by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, A-549, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP16 antibody (Product # A305-805A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RanBP16 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP16 antibody (Product # A305-805A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse RanBP16 by western blot. Samples: Whole cell lysate (15 µg) from Jurkat, A-549, Hep-G2, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP16 antibody A305-805A-M (lot A305-805A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RanBP16 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanBP16 antibody (Product # A305-805A-T; lot 2) used for IP at 2 µg per reaction. RanBP16 was also immunoprecipitated by a previous lot of this antibody (Product # A305-805A-T; lot 1) and a second antibody against a different epitope of RANBP16 (BL23431). For blotting immunoprecipitated RanBP16, Product # A305-805A-T was used at 1:40. Detection: Chemiluminescence with an exposure time of 1 second.