PA5-27945

antibody from Invitrogen Antibodies

Targeting: PLA2G6 iPLA2, iPLA2beta, NBIA2, PARK14, PNPLA9

Antibody data

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Validation data

Product number: PA5-27945 - Provider product page
Provider: Invitrogen Antibodies
Product name: PLA2G6 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: U87-MG. Predicted reactivity: Mouse (94%), Rat (93%), Rabbit (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.74 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PLA2G6 protein structure - PA5-27945 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1. Effect of point mutation on iPLA 2 beta function. a , Time course of the formation of 1-SA-OH-PE in reactions catalyzed by iPLA 2 beta R747W mutant (red circles) or WT iPLA 2 beta (black circles). 1-SA-2-ETE-PE ( left ) and 1-SA-2-15-HpETE-PE ( right ) were used as substrates. Data are means+-s.d., *p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5. Homozygous Pnpla9 R748W leads to Parkinsonian phenotype and ferroptotic oxygenated PE accumulation in midbrain of mice. a, Time (sec) taken to climb down the pole, and b, time (sec) on the rotarod for WT and Pnpla9 R748W mutant mice at 2-7 months of age. #### p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Extended Data Fig. 8 iPLA 2 beta-deficient cells are more sensitive to RSL3-induced ferroptosis compared to WT cells. a, Total PLA 2 activity in H109 and fPD R747W cells in the absence and in the presence of (S)-BEL. Cell supernatants were incubated with 1-SA-2-ETE-PE (upper panel) or 1-SA-2-15-HpETE-PE (lower panel) for 30 min at 37degC. Activity is presented as 1-SA-2-OH-PE, pmol/min/mg protein. The background levels of 1-SA-2-OH-PE in H109 and fPD R747W cell supernatants were low and estimated as 2.76+-0.19 and 2.74+-0.75 pmols per sample vs 70.5 +- 8.5 and 100.7 +- 10.5 pmols per sample accumulated in H109 and fPD R747W cell supernatants during incubation in the absence of S-BEL. Data are means +- s.d., *p=0.0014 for H109 cells, **p= 0.0073 for fPD R743w cells, ****p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Extended Data Fig. 10 Content of PE, oxygenated PE in midbrain of rotenone exposed rats and 8-months old WT and A53T mice. a, Content of PE in substantia nigra of control rats (treated with vehicle, DMSO) and Parkinsonian rats (treated with rotenone for 14 days at a dose of 3mg/kg/day). Data are presented as pmol/nmol of total phospholipids, N=6 biologically independent animals. d-diacyl species; p-alkenyl (plasmalogen) species. b , Quantification of oxygenated PE species in substantia nigra of control rats (treated with vehicle, DMSO) and Parkinsonian rats (treated with rotenone for 14 days at the dose of 3mg/kg/day). Data presented as pmol/mmol of total phospholipids, N=6 biologically independent animals. c , iPLA 2 beta protein expression in midbrain of 8-months old WT and A53T mice. Inset: Typical western blot of iPLA 2 beta. Data are means +- SD, ****p