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LearnMA5-22212
antibody from Invitrogen Antibodies
Targeting: CENPJ
BM032, CPAP, LAP, LIP1, MCPH6, Sas-4, SASS4, SCKL4
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- MA5-22212 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CENPJ Monoclonal Antibody (1A5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: KYTTAARTFP DKKEREEIQT LKQQIADLRE DLKRKETKWS STHSRLRSQI QMLVRENTDL REEIKVMERF RLDAWKRAEA IESSLEVEKK DKLANTSVRF QNSQISSGTQ
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1A5
- Vial size
- 50 µg
- Concentration
- 0.2-1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CENPJ expressed in transfected 293T cell line by anti-CENPJ monoclonal antibody (Product # MA5-22212), clone 1A5. Lane 1: CENPJ transfected lysate (43.2 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CENPJ using anti-CENPJ monoclonal antibody (Product # MA5-22212) is 0.1 ng/mL as a capture antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CENPJ transfected lysate using anti-CENPJ monoclonal antibody (Product # MA5-22212) and Protein A Magnetic Beads, and immunoblotted with CENPJ rabbit polyclonal antibody.
