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LearnTA504509S
antibody from Invitrogen Antibodies
Targeting: FOLH1
FOLH, GCP2, GCPII, NAALAD1, NAALAdase, PSM, PSMA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA504509S - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOLH1 Monoclonal Antibody (OTI2E1), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2E1
- Vial size
- 30 µL
- Concentration
- 0.84 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOLH1 (RC218310, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FOLH1. Positive lysates LY429203 (100 µg) and LC429203 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOLH1 (RC218310, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FOLH1. Positive lysates LY429203 (100 µg) and LC429203 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-FOLH1 monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 9 Human tissue by using anti-FOLH1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Standard curve for ELISA analysis with FOLH1 recombinant protein (dilution range from 5 ng/mL to 5µg/ml) using FOLH1 Capture antibody (Cat# TA504509) at 5µg/ml and HRP conj µgated FOLH1 Detection mAb (Cat# TA504550) at 0.03µg/
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-FOLH1 mouse monoclonal antibody (TA504509) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOLH1(RC218310).
