LS-C777096

antibody from LSBio

Targeting: AXIN1 PPP1R49

Western blot (Enhanced validation)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C777096

Provider

LSBio

Product name

AXIN1 / Axin-1 Antibody (APC) LS-C777096

Antibody type

Polyclonal

Description

Peptide Affinity Purified

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Red dye

Storage

Store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Rat Kidney showing detection of ~95 kDa Axin-1 protein using Rabbit Anti-Axin-1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-1 Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95 kDa.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody. Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody. Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody. Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody. Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis using Rabbit Anti-Axin 1 Polyclonal Antibody. Tissue: Breast. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Axin 1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.