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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-41161 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRDM9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: CPGDQNQEQQ YPDPHSRNDK TKGQEIKERS KLLNKRTWQR EISRAFSSPP Sequence homology: Human: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Depletion of PRDM9 enhances proliferation, migration and chemotaxis potentials in human periodontal ligament stem cells.
Zhang J, Zhang C, Yang H, Han X, Fan Z, Hou B
Connective tissue research 2020 Sep;61(5):498-508
Connective tissue research 2020 Sep;61(5):498-508
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 cells using an anti-PRDM9 polyclonal antibody (Product # PA5-41161).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human 293T cell lysate using an anti-PRDM9 polyclonal antibody (Product # PA5-41161).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human skeletal muscle tissue using an anti-PRDM9 polyclonal antibody (Product # PA5-41161).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human testis tissue using an anti-PRDM9 polyclonal antibody (Product # PA5-41161).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human placenta tissue using an anti-PRDM9 polyclonal antibody (Product # PA5-41161).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Depletion of PRDM9 enhanced the proliferation of PDLSCs and regulated the cell cycle. ( A ) Western blot analysis showed depletion of PRDM9. ( B ) The CCK-8 assay revealed that depletion of PRDM9 increased cell numbers at 24 h and 48 h. ( C ) Cell doubling times. ( D, E ) Flow cytometric cell cycle analysis showed a markedly lower percentage of G0/G1 phase cells and an obviously higher percentage of S phase and G2/M phase cells in the PRDM9sh group. ( F ) Proliferation index. Student's t test was employed to test statistical significance. All error bars represent SD (n = 3). *P
