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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ5719 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Ribonuclease 3 Antibody / DROSHA / RNase III
- Antibody type
- Polyclonal
- Description
- This highly specific Ribonuclease 3 antibody is suitable for use in Western blot/Direct ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Ribonuclease 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) HEK293, 3) Jurkat, 4) HepG2, 5) SW620, 6) K562, 7) mouse Neuro-2a and 8) mouse ANA-1 lysate with Ribonuclease 3 antibody. Predicted molecular weight ~159 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HL-60 cells with Ribonuclease 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease 3 antibody.
