MA5-14784
antibody from Invitrogen Antibodies
Targeting: DROSHA
Etohi2, HSA242976, RN3, RNASE3L, RNASEN
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- MA5-14784 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Drosha Monoclonal Antibody (S.12.0)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- S.12.0
- Vial size
- 100 µL
- Concentration
- 615 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Drosha in extracts from Hela and K562 cells using Drosha monoclonal antibody (Product # MA5-14784).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Drosha was achieved by transfecting HeLa cells with Drosha specific siRNAs (Silencer® select Product # s26492, s26491). Western blot analysis (Fig. a) was performed using whole cell extracts from the Drosha knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Drosha Monoclonal Antibody (S.12.0) (Product # MA5-14784, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Drosha. An uncharacterized band was observed around ~50KDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), C2C12 (Lane 3) and K-562 (Lane 4). The blot was probed with anti-Drosha Monoclonal Antibody (Product # MA5-14784, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 180 kDa band corresponding to Drosha was detected across the cell lines tested.