PA5-90076

antibody from Invitrogen Antibodies

Targeting: ERLIN1 C10orf69, Erlin-1, KE04, SPFH1, SPG62

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-90076

Provider

Invitrogen Antibodies

Product name

ERLIN1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KADAEYYAAH KYATSNKHKL TPEYLELKKY QAIASNSKIY FGSNIPNMFV DSSCALKYSD IRTGRESSLP SKEALEPSGE NVIQNKESTG; Positive Samples: 293T, HepG2, BxPC3, DU145, OVCAR-3, Mouse kidney, Rat testis; Cellular Location: Endoplasmic reticulum membrane, Single-pass type II membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.26 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of ERLIN1 in 300 μg extracts of DU145 cells. Samples were precipitated with 3 μg ERLIN1 Polyclonal antibody (Product # PA5-90076). Western blot was performed from the immunoprecipitate using ERLIN1 Polyclonal antibody (Product # PA5-90076) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ERLIN1 in paraffin-embedded human colon. Samples were incubated with ERLIN1 Polyclonal antibody (Product # PA5-90076) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.