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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-51539 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UNC84B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LKSEWQSMTQ ESFQESSVKE LRRLEDQLAG LQQELAALAL KQSSVAEEVG LLPQQIQAVR DDVESQFPAW ISQFLARGGG GRVGLLQREE MQAQLRELES KILTHVAEMQ GKSAREAAAS LSLTLQKEGV IGVTEEQVHH IVKQALQRYS E
- Concentration
- 0.1 mg/mL
Submitted references Cytoplasmic control of intranuclear polarity by human cytomegalovirus.
Procter DJ, Furey C, Garza-Gongora AG, Kosak ST, Walsh D
Nature 2020 Nov;587(7832):109-114
Nature 2020 Nov;587(7832):109-114
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UNC84B in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using a UNC84B Polyclonal Antibody (Product # PA5-51539). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of UNC84B was achieved by transfecting A-431 with UNC84B specific siRNAs (Silencer® select Product # s24465, s24466). Western blot analysis (Fig. a) was performed using whole cell extracts from the WAVE2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with UNC84B Polyclonal Antibody (Product # PA5-51539, 0.4 ug/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to UNC84B.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-UNC84B Polyclonal Antibody (Product # PA5-51539) and a 80 kDa band corresponding to UNC84B was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), Jurkat (Lane 4) and A549 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 ug/ml) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of UNC84B in human cell line A-431 shows positivity in nuclear membrane. Samples were probed using an UNC84B Polyclonal Antibody (Product # PA5-51539).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of UNC84B was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with UNC84B Rabbit Polyclonal Antibody (Product # PA5-51539) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear and Nuclear membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UNC84B in human cerebral cortex using an UNC84B Polyclonal Antibody (Product # PA5-51539) shows moderate to strong positivity in nuclear membrane in neuronal cells and glial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UNC84B in human cerebral cortex using an UNC84B Polyclonal Antibody (Product # PA5-51539) shows moderate to strong positivity in nuclear membrane in neuronal cells and glial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UNC84B in human testis using an UNC84B Polyclonal Antibody (Product # PA5-51539) shows moderate to strong positivity in nuclear membrane in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UNC84B in human stomach using an UNC84B Polyclonal Antibody (Product # PA5-51539) shows moderate to strong positivity in nuclear membrane in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UNC84B in human kidney using an UNC84B Polyclonal Antibody (Product # PA5-51539) shows moderate to strong positivity in nuclear membrane in cells in tubules and cells in glomeruli.
