A300-954A

antibody from Invitrogen Antibodies

Targeting: DOT1L DOT1, KIAA1814, KMT4

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-954A

Provider

Invitrogen Antibodies

Product name

DOT1L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DOT1L by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody A300-954A used for IP at 3 µg/mg lysate. DOT1L was also immunoprecipitated by rabbit anti-DOT1L antibody A300-953A, which recognizes an upstream epitope. For blotting immunoprecipitated DOT1L, A300-953A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time 10 seconds.