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- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- UM800031 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800031, RRID:AB_2629143
- Product name
- CD23 (FCER2) mouse monoclonal antibody,clone UMAB101
- Antibody type
- Monoclonal
- Description
- CD23 (FCER2) mouse monoclonal antibody,clone UMAB101
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FCER2
- Isotype
- IgG
- Antibody clone number
- UMAB101
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Jurkat cells using anti-CD23 mouse monoclonal antibody (UM800031, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20?m.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FCER2 mouse monoclonal antibody. (UM800031; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using FCER2 (CD23) clone UMAB101, mouse monoclonal antibody at 1:400 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800031 requires heat-induced epitope retrieval with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image is a composite of 3 tonsils which show strong membranous and cytoplasmic staining however each tonsil has a unique expression pattern in the inner and outer germinal center.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human spleen using FCER2 (CD23) clone UMAB101, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800031 requires heat-induced epitope retrieval with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image is a composite of 3 spleens which show strong membranous and cytoplasmic staining white pulp.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-FCER2 (CD23) clone UMAB101 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, UM800031)(1:500).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD23 clone UMAB101 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM800031)(1:500)
- Validation comment
- IHC
