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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [6]
- Flow cytometry [1]
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- Product number
- R31482 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD23 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific CD23 antibody is suitable for use in Western blot/Immunohistochemistry/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the CD23 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of CD23 antibody and mouse liver lysate. Expected size 37~45KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of CD23 antibody and 1) rat spleen and 2) rat thymus lysate. Expected molecular weight: 37~45 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: CD23 antibody testing of human tonsil tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: CD23 antibody testing of mouse spleen tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: CD23 antibody testing of rat spleen tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE mouse lymph tissue with CD23 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE rat lymph tissue with CD23 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of frozen mouse spleen tissue with CD23 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse PBMs with CD23 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD23 antibody.
