H00002208-M14

antibody from Abnova Corporation

Targeting: FCER2 CD23, CD23A, CLEC4J, FCE2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002208-M14

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002208-M14, RRID:AB_1136932

Product name

FCER2 monoclonal antibody (M14), clone 2A7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant FCER2.

Antigen sequence

MEEGQYSEIEELPRRRCCRRGTQIVLLGLVTAALW
AGLLTLLLLWHWDTTQGLKQLEERAARNVSQVSKN
LESHHGDQMAQKSQSTQISQELEELRAEQQRLKSQ
DLELSWNLNGLQADLSSFKSQELNERNEASDLLER
LREEVTKLRMELQVSSGFVCNTCPEKWINFQRKCY
YFGKGTKQWVHARYACDDMEGQLVSIHSPEEQDFL
TKHASHTGSWIGLRNLDLKGEFIWVDGSHVDYSNW
APGEPTSRSQGEDCVMMRGSGRWNDAFCDRKLGAW
VCDRLATCTPPASEGSAESMGPDSRPDPDGRLPTP
SAPLHS


Isotype

IgG

Antibody clone number

2A7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FCER2 expression in transfected 293T cell line by FCER2 monoclonal antibody (M14), clone 2A7.Lane 1: FCER2 transfected lysate(36.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FCER2 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol