Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-0238-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD23 Monoclonal Antibody (EBVCS2), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The EBVCS2 monoclonal antibody reacts with human CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on mature B cells, mantle zone B cells, follicular dendritic cells and at low levels on T, NK, langerhans cells and platelets. Expression of CD23 is upregulated upon B cell activation, and soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B cell activation. CD21 and the alpha subunit of CD11b and CD11c bind to CD23. Applications Reported: The EBVCS2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This EBVCS2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- EBVCS2
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.
CAY10593 inhibits the human P2X7 receptor independently of phospholipase D1 stimulation.
Interleukin-25 fails to activate STAT6 and induce alternatively activated macrophages.
Balan S, Ollion V, Colletti N, Chelbi R, Montanana-Sanchis F, Liu H, Vu Manh TP, Sanchez C, Savoret J, Perrot I, Doffin AC, Fossum E, Bechlian D, Chabannon C, Bogen B, Asselin-Paturel C, Shaw M, Soos T, Caux C, Valladeau-Guilemond J, Dalod M
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1622-35
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):1622-35
CAY10593 inhibits the human P2X7 receptor independently of phospholipase D1 stimulation.
Pupovac A, Stokes L, Sluyter R
Purinergic signalling 2013 Dec;9(4):609-19
Purinergic signalling 2013 Dec;9(4):609-19
Interleukin-25 fails to activate STAT6 and induce alternatively activated macrophages.
Stolfi C, Caruso R, Franzè E, Sarra M, De Nitto D, Rizzo A, Pallone F, Monteleone G
Immunology 2011 Jan;132(1):66-77
Immunology 2011 Jan;132(1):66-77
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD23 PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye