V4592

antibody from NSJ Bioreagents

Targeting: FCER2 CD23, CD23A, CLEC4J, FCE2

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V4592

Provider

NSJ Bioreagents

Product name

CD23 Antibody

Antibody type

Monoclonal

Description

This highly specific CD23 antibody is suitable for use in Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

FCER2/6893

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the CD23 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human tonsil. Membrane stained using FCER2/6893 at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD23 antibody (clone FCER2/6893). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free CD23 antibody (clone FCER2/6893) as confirmation of integrity and purity.