- MA5-14572 - Invitrogen Antibodies FCER2 antibody

Submitted references Follicular dendritic cell sarcoma: clinicopathologic study of 15 cases with emphasis on novel expression of MDM2, somatostatin receptor 2A, and PD-L1.

Agaimy A, Michal M, Hadravsky L, Michal M
Annals of diagnostic pathology 2016 Aug;23:21-8

A reference image-based method for optimization of clinical immunohistochemistry.

Kap M, Lam KH, Ewing-Graham P, Riegman P
Histopathology 2015 Aug;67(2):193-205

Inadequate T follicular cell help impairs B cell immunity during HIV infection.

Cubas RA, Mudd JC, Savoye AL, Perreau M, van Grevenynghe J, Metcalf T, Connick E, Meditz A, Freeman GJ, Abesada-Terk G Jr, Jacobson JM, Brooks AD, Crotty S, Estes JD, Pantaleo G, Lederman MM, Haddad EK
Nature medicine 2013 Apr;19(4):494-9

Histological assessment of PAXgene tissue fixation and stabilization reagents.

Kap M, Smedts F, Oosterhuis W, Winther R, Christensen N, Reischauer B, Viertler C, Groelz D, Becker KF, Zatloukal K, Langer R, Slotta-Huspenina J, Bodo K, de Jong B, Oelmuller U, Riegman P
PloS one 2011;6(11):e27704

Simultaneous activity assay of two transglutaminase isozymes, blood coagulation factor XIII and transglutaminase 2, by use of fibrinogen arrays.

Kwon MH, Jung SH, Kim YM, Ha KS
Analytical chemistry 2011 Nov 15;83(22):8718-24

Composite biclonal marginal zone lymphoma of lung and chronic lymphocytic leukemia: pathologic, phenotypic, cytogenetic, and molecular study.

Bhagavathi S, Micale MA, Douglas-Nikitin V, Ballouz S, Neumann K, Blenc AM
International journal of surgical pathology 2011 Jun;19(3):377-81

The matricellular protein SPARC supports follicular dendritic cell networking toward Th17 responses.

Piconese S, Costanza M, Tripodo C, Sangaletti S, Musio S, Pittoni P, Poliani PL, Burocchi A, Passafaro AL, Gorzanelli A, Vitali C, Chiodoni C, Barnaba V, Pedotti R, Colombo MP
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Pediatric lymphomas in Brazil.

Gualco G, Klumb CE, Barber GN, Weiss LM, Bacchi CE
Clinics (Sao Paulo, Brazil) 2010;65(12):1267-77

Pediatric lymphomas in Brazil.

Gualco G, Klumb CE, Barber GN, Weiss LM, Bacchi CE
Clinics (Sao Paulo, Brazil) 2010;65(12):1267-77

Cyclin D1 expression in B-cell lymphomas.

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Experimental hematology 2010 Nov;38(11):1047-57

Sarcoma of follicular dendritic cells with features of sinus lining cells--a new subtype of reticulum cell sarcoma?

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Virchows Archiv : an international journal of pathology 2008 May;452(5):565-70

Burkitt lymphoma in Brazil is characterized by geographically distinct clinicopathologic features.

Queiroga EM, Gualco G, Weiss LM, Dittmer DP, Araujo I, Klumb CE, Harrington WJ Jr, Bacchi CE
American journal of clinical pathology 2008 Dec;130(6):946-56

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD23 was performed by loading 25 µg of Raji (Lane 1), Daudi (Lane 2) and BAF-3 (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD23 monoclonal antibody (Product # MA5-14572) at a dilution of 1:20 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 45 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human tonsil stained with CD23 using AEC chromogen. Note membrane staining of B-cells and dendritic cell

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD23 in BAF-3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD23 monoclonal antibody (Product # MA5-14572) at a dilution of 1:2 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD23 in peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-14572) at a dilution of 1:2. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD23 in peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-14572) at a dilution of 1:2. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.

MA5-14572

Antibody data