MA5-16335

antibody from Invitrogen Antibodies

Targeting: FCER2 CD23, CD23A, CLEC4J, FCE2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA5-16335

Provider

Invitrogen Antibodies

Product name

CD23 Monoclonal Antibody (SP23)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

SP23

Vial size

500 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Follicular dendritic cell sarcoma: clinicopathologic study of 15 cases with emphasis on novel expression of MDM2, somatostatin receptor 2A, and PD-L1.

Agaimy A, Michal M, Hadravsky L, Michal M
Annals of diagnostic pathology 2016 Aug;23:21-8

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Inadequate T follicular cell help impairs B cell immunity during HIV infection.

Cubas RA, Mudd JC, Savoye AL, Perreau M, van Grevenynghe J, Metcalf T, Connick E, Meditz A, Freeman GJ, Abesada-Terk G Jr, Jacobson JM, Brooks AD, Crotty S, Estes JD, Pantaleo G, Lederman MM, Haddad EK
Nature medicine 2013 Apr;19(4):494-9

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Pediatric lymphomas in Brazil.

Gualco G, Klumb CE, Barber GN, Weiss LM, Bacchi CE
Clinics (Sao Paulo, Brazil) 2010;65(12):1267-77

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Pediatric lymphomas in Brazil.

Gualco G, Klumb CE, Barber GN, Weiss LM, Bacchi CE
Clinics (Sao Paulo, Brazil) 2010;65(12):1267-77

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Cyclin D1 expression in B-cell lymphomas.

Gladkikh A, Potashnikova D, Korneva E, Khudoleeva O, Vorobjev I
Experimental hematology 2010 Nov;38(11):1047-57

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Raji Cells using anti-CD23 Monoclonal Antibody (Product # MA5-16335). The recommened dilution for this antibody in western blot applications is 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CD23 using anti-CD23 Monoclonal Antibody (Product # MA5-16335) in Tonsil Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.