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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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- Product number
- AF123 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD23/Fc epsilon RII Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD23/Fc epsilon RII in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P06734- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cytoplasmic myosin-exposed apoptotic cells appear with caspase-3 activation and enhance CLL cell viability.
Functional impact of NOTCH1 mutations in chronic lymphocytic leukemia.
Cui X, Zhang L, Magli AR, Catera R, Yan XJ, Griffin DO, Rothstein TL, Barrientos J, Kolitz JE, Allen SL, Rai KR, Chiorazzi N, Chu CC
Leukemia 2016 Jan;30(1):74-85
Leukemia 2016 Jan;30(1):74-85
Functional impact of NOTCH1 mutations in chronic lymphocytic leukemia.
Arruga F, Gizdic B, Serra S, Vaisitti T, Ciardullo C, Coscia M, Laurenti L, D'Arena G, Jaksic O, Inghirami G, Rossi D, Gaidano G, Deaglio S
Leukemia 2014 May;28(5):1060-70
Leukemia 2014 May;28(5):1060-70
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- CD23/Fc epsilon RII in Human PBMCs. CD23/Fc epsilon RII was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CD23/Fc epsilon RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
