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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-35851 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HepG2. Predicted reactivity: Mouse (96%), Rat (96%), Dog (98%), Pig (98%), Sheep (98%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Poorly-Controlled Type 1 Diabetes Mellitus Impairs LH-LHCGR Signaling in the Ovaries and Decreases Female Fertility in Mice.
Lee J, Lee HC, Kim SY, Cho GJ, Woodruff TK
Yonsei medical journal 2019 Jul;60(7):667-678
Yonsei medical journal 2019 Jul;60(7):667-678
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AMH Polyclonal Antibody detects AMH protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.7.5% SDS-PAGE. AMH Polyclonal Antibody (Product # PA5-35851) dilution: 1:2,500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AMH Polyclonal Antibody detects AMH protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: AMH protein stained by AMH Polyclonal Antibody (Product # PA5-35851) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AMH Polyclonal Antibody detects AMH protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: AMH protein stained by AMH Polyclonal Antibody (Product # PA5-35851) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AMH Polyclonal Antibody detects AMH protein at cytosol on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. AMH Polyclonal Antibody (Product # PA5-35851) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AMH Polyclonal Antibody detects AMH protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. AMH Polyclonal Antibody (Product # PA5-35851) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
