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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PA5-37899 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNNT3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Troponin T/TNNT3 in (A) human (B) mouse and (C) rat skeletal muscle lysates was performed using 35 µg protein in RIPA buffer. Lysates were probed with a Troponin T/TNNT3 polyclonal antibody (Product # PA5-37899) at a dilution of 0.01 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TNNT3 in paraffin embedded human skeletal muscle using a TNNT3 polyclonal antibody (Product #PA5-37899) at a concentration of 5 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Troponin T/TNNT3 in paraffin-embedded human skeletal muscle following steamed antigen retrieval with a citrate buffer, pH 6, using a Troponin T/TNNT3 polyclonal antibody (Product # PA5-37899) at a dilution of 5 µg/mL. Detection was performed using an AP-conjugated secondary antibody.
