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antibody from Invitrogen Antibodies
Targeting: NSFL1C
dJ776F14.1, p47, UBX1, UBXD10, UBXN2C
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-21633 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSFL1C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (83%), Pig (98%), Chicken (87%), Rhesus Monkey (100%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation.
Shih YT, Hsueh YP
Nature communications 2016 Mar 17;7:11020
Nature communications 2016 Mar 17;7:11020
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of DU145 (Lane 1), SKOV3 (Lane 2), HepG2 (Lane 3), PC12 (Lane 4), MKN45 (Lane 5), HeLa (Lane 6), SHSY5Y (Lane 7), Jurkat (Lane 8), and A431 (Lane 9). The blot was probed with Anti-NSFL1C Polyclonal Antibody (Product # PA5-21633, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 47 kDa band corresponding to NSFL1C was observed in all the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NSFL1C Polyclonal Antibody (Product # PA5-21633). Sample (30 µg whole cell lysate). A: 293T. B: A431. C: H1299. 10% SDS PAGE. NSFL1C Polyclonal Antibody (Product # PA5-21633) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NSFL1C Polyclonal Antibody (Product # PA5-21633). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. NSFL1C Polyclonal Antibody (Product # PA5-21633) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of NSFL1C was achieved by transfecting A431 cells with NSFL1C specific siRNAs (Silencer® select Product # s31818, s31817). Western blot analysis (Fig. a) was performed using whole cell extracts from the NSFL1C knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with NSFL1C Polyclonal Antibody (Product # PA5-21633, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NSFL1C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NSFL1C was performed in paraffin-embedded mouse brain tissue using NSFL1C Polyclonal Antibody (Product # PA5-21633) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded differential N/T pair Lung SCC cancer xenograft, using NSFL1C (Product # PA5-21633) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NSFL1C was performed in A431 whole cell extracts using 5 µg of NSFL1C Polyclonal Antibody (Product # PA5-21633). Samples were transferred to a membrane and probed with NSFL1C Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
