200-301-A87

antibody from Rockland Immunochemicals, Inc.

Targeting: MSLN CAK1, MPF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-301-A87

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-301-A87, RRID:AB_2235614

Product name

Anti-Mesothelin (MOUSE) Monoclonal Antibody - 200-301-A87

Antibody type

Monoclonal

Vial size

100 µl

References

Submitted references

Diffuse mesothelin expression leads to worse prognosis through enhanced cellular proliferation in colorectal cancer.

Inoue S, Tsunoda T, Riku M, Ito H, Inoko A, Murakami H, Ebi M, Ogasawara N, Pastan I, Kasugai K, Kasai K, Ikeda H, Inaguma S
Oncology letters 2020 Mar;19(3):1741-1750

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Combining Local Immunotoxins Targeting Mesothelin with CTLA-4 Blockade Synergistically Eradicates Murine Cancer by Promoting Anticancer Immunity.

Leshem Y, O'Brien J, Liu X, Bera TK, Terabe M, Berzofsky JA, Bossenmaier B, Niederfellner G, Tai CH, Reiter Y, Pastan I
Cancer immunology research 2017 Aug;5(8):685-694

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Malignant Mesothelioma Effusions Are Infiltrated by CD3(+) T Cells Highly Expressing PD-L1 and the PD-L1(+) Tumor Cells within These Effusions Are Susceptible to ADCC by the Anti-PD-L1 Antibody Avelumab.

Khanna S, Thomas A, Abate-Daga D, Zhang J, Morrow B, Steinberg SM, Orlandi A, Ferroni P, Schlom J, Guadagni F, Hassan R
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2016 Nov;11(11):1993-2005

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Reduced Shedding of Surface Mesothelin Improves Efficacy of Mesothelin-Targeting Recombinant Immunotoxins.

Awuah P, Bera TK, Folivi M, Chertov O, Pastan I
Molecular cancer therapeutics 2016 Jul;15(7):1648-55

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Mesothelin Expression in Advanced Gastroesophageal Cancer Represents a Novel Target for Immunotherapy.

Illei PB, Alewine C, Zahurak M, Cowan ML, Montgomery E, Hassan R, Xiang L, Pastan I, Kelly RJ
Applied immunohistochemistry & molecular morphology : AIMM 2016 Apr;24(4):246-52

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Megakaryocytic potentiating factor and mature mesothelin stimulate the growth of a lung cancer cell line in the peritoneal cavity of mice.

Zhang J, Bera TK, Liu W, Du X, Alewine C, Hassan R, Pastan I
PloS one 2014;9(8):e104388

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Efficacy of RG7787, a next-generation mesothelin-targeted immunotoxin, against triple-negative breast and gastric cancers.

Alewine C, Xiang L, Yamori T, Niederfellner G, Bosslet K, Pastan I
Molecular cancer therapeutics 2014 Nov;13(11):2653-61

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Loss of mesothelin expression by mesothelioma cells grown in vitro determines sensitivity to anti-mesothelin immunotoxin SS1P.

Zhang J, Qiu S, Zhang Y, Merino M, Fetsch P, Avital I, Filie A, Pastan I, Hassan R
Anticancer research 2012 Dec;32(12):5151-8

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blotting using Rockland's anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1 ?g/ml followed by reaction with ALP goat anti-mouse IgG and BCIP/NBT substrate. Reprinted with permission fromClin.Cancer Res. 11(16):5840-6.

Validation comment

Western Blot