MA5-17041

antibody from Invitrogen Antibodies

Targeting: ENG CD105, END, HHT1, ORW, ORW1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17041

Provider

Invitrogen Antibodies

Product name

CD105 Monoclonal Antibody (3A9)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17041 targets CD105 in indirect ELISA, FACS, ICC, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-17041 immunogen is purified recombinant fragment of human CD105 expressed in E. Coli. MA5-17041 detects CD105 which has a predicted molecular weight of approximately 71kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3A9

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

A study to identify and characterize the stem/progenitor cell in rabbit meniscus.

Huang H, Wang S, Gui J, Shen H
Cytotechnology 2016 Oct;68(5):2083-103

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Endoglin, VEGF, and its receptors in predicting metastases in endometrial carcinoma.

Saarelainen SK, Staff S, Peltonen N, Lehtimäki T, Isola J, Kujala PM, Vuento MH, Mäenpää JU
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 May;35(5):4651-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD105/endoglin using CD105/endoglin monoclonal antibody (Product # MA5-17041) in HEK293 (1) and CD105 (AA: 331-567) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using CD105 Monoclonal Antibody (3A9) (Product # MA5-17041) and a ~70 kDa band corresponding to CD105 was observed in HUVEC, HMEC-1, T98G and HeLa, as reported. Whole cell extracts (30 µg lysate) of HUVEC (Lane 1), HMEC-1 (Lane 2), T98G (Lane 3), U-87 MG (Lane 4), HeLa (Lane 5), HEK-293 (Lane 6), SK-O-V3 (Lane 7), LNCaP (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 cells using CD105/endoglin monoclonal antibody (Product # MA5-17041) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 cells using CD105/endoglin monoclonal antibody (Product # MA5-17041) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105/endoglin monoclonal antibody (Product # MA5-17041) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using CD105/endoglin monoclonal antibody (Product # MA5-17041) (green) and negative control (purple).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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