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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- 102-PA60S - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- CD105/Endoglin
- Antibody type
- Polyclonal
- Antigen
- Recombinant human sCD105/Endoglin
- Description
- antibody Protein-A purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
ETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNA
ILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREV
LLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEP
PGVNTTELPSFPKTQILEWAAERGPITSAAELNDP
QSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRT
PALVRGCHLEGVAGHKEAHILRVLPGHSAGPRTVT
VKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQ
IWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARM
LNASIVASFVELPLASIVSLHASSCGGRLQTSPAP
IQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKE
LVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYS
SCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNM
DSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVS
PSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGN
CVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCT
VALRPKTGSQDQEVHRTVFMRLNIISPDLSGCTSH
HHHHH- Antibody clone number
- Rabbit IG
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type
Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas
Steiniger B, Wilhelmi V, Seiler A, Lampp K, Stachniss V
Immunology 2014 November;143(3):462-477
Immunology 2014 November;143(3):462-477
Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas
Norgall S, Papoutsi M, Rössler J, Schweigerer L, Wilting J, Weich H
BMC Cancer 2007 December;7(1)
BMC Cancer 2007 December;7(1)
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western analysis of recombinant human [Cat# S01-024] and human [Cat# S01-022] soluble CD105 using an anti-human CD105 antibody [Cat# 102-PA60] directed against recombinant human and mouse soluble CD105 produced in insect cells. The SDS-PAGE was run under reducing conditions. There is a strong cross reaction between human and mouse visible.
- Sample type
- Purified recombinant proteins
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Double staining of human colon and colon carcinoma tissue with Endoglin (Cat# 102-PA60) / CD31. The experiments were perfomed by Dr. Ulrike Fiedler and Stefanie Koidel, Dept. of Vascular Biology and Angiogenesis Research, Tumor Biology Center, Breisacher Str. 117, D-79106 Freiburg, Germany
- Sample type
- Human colon carcinoma tissue
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with human umbilical vein endothelial cells (HUVEC).
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
