TA506170

antibody from OriGene

Targeting: ENG CD105, END, HHT1, ORW, ORW1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA506170

Provider

OriGene

Proper citation

OriGene Cat#TA506170, RRID:AB_2623660

Product name

ENG (CD105) mouse monoclonal antibody, clone OTI8A1 (formerly 8A1)

Antibody type

Monoclonal

Description

ENG (CD105) mouse monoclonal antibody, clone OTI8A1 (formerly 8A1)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

ENG

Isotype

IgG

Antibody clone number

OTI8A1

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENG (RC221699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENG.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ENG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ENG monoclonal antibody.(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody (TA506170).

Validation comment

IF