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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- DP3517P - Provider product page
- Provider
- OriGene
- Product name
- CD105 (ENG) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- CD105 (ENG) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ENG
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- Lyophilized
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western analysis of recombinant human ( DA3522) and human ( DA3523) soluble CD105 using an anti-Human CD105 antibody (?) directed against recombinant human soluble CD105 produced in insect cells. The SDS-PAGE was run under reducing conditions. There is a strong cross reaction between human and mouse visible.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence staining (green) of CD105 in primary human dermal lymphatic endothelial cells (HDLEC) with anti-Human CD105 (0.5ug/ml) and counter staining of nuclei with Dapi. As secondary antibody goat anti-rabbit ALEXA Flour 488 was used 1:400.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FACS analysis with primary human umbilical vein endothelial cells?(HUVEC).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
- Validation comment
- FC
