MA5-11854

antibody from Invitrogen Antibodies

Targeting: ENG CD105, END, HHT1, ORW, ORW1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-11854

Provider

Invitrogen Antibodies

Product name

Anti-CD105 Monoclonal Antibody (SN6h)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-11854 targets CD105 in FACS, IF, IHC (P), and WB applications and shows reactivity with Human and Bovine samples. The MA5-11854 immunogen is purified GP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells. MA5-11854 was used in the immunofluorescence analysis to successfully detect CD105 in human mesenchymal stem cells derived from iPSCs.

Reactivity

Human, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

SN6h

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Genetically modified human placenta‑derived mesenchymal stem cells with FGF‑2 and PDGF‑BB enhance neovascularization in a model of hindlimb ischemia.

Yin T, He S, Su C, Chen X, Zhang D, Wan Y, Ye T, Shen G, Wang Y, Shi H, Yang L, Wei Y
Molecular medicine reports 2015 Oct;12(4):5093-9

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PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.

Yuan SM, Guo Y, Zhou XJ, Shen WM, Chen HN
International journal of clinical and experimental pathology 2014;7(6):2861-70

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Angiogenesis and expression of PDGF-C, VEGF, CD105 and HIF-1α in human glioblastoma.

Clara CA, Marie SK, de Almeida JR, Wakamatsu A, Oba-Shinjo SM, Uno M, Neville M, Rosemberg S
Neuropathology : official journal of the Japanese Society of Neuropathology 2014 Aug;34(4):343-52

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Mesenchymal-to-endothelial transition in Kaposi sarcoma: a histogenetic hypothesis based on a case series and literature review.

Gurzu S, Ciortea D, Munteanu T, Kezdi-Zaharia I, Jung I
PloS one 2013;8(8):e71530

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Intravascular papillary endothelial hyperplasia: histomorphological and immunohistochemical features.

Akdur NC, Donmez M, Gozel S, Ustun H, Hucumenoglu S
Diagnostic pathology 2013 Oct 14;8:167

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HGF-transgenic MSCs can improve the effects of tissue self-repair in a rabbit model of traumatic osteonecrosis of the femoral head.

Wen Q, Jin D, Zhou CY, Zhou MQ, Luo W, Ma L
PloS one 2012;7(5):e37503

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Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies.

Nolan-Stevaux O, Zhong W, Culp S, Shaffer K, Hoover J, Wickramasinghe D, Ruefli-Brasse A
PloS one 2012;7(12):e50920

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Mesenchymal stem cells in infantile hemangioma reside in the perivascular region.

Yuan SM, Chen RL, Shen WM, Chen HN, Zhou XJ
Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society 2012 Jan-Feb;15(1):5-12

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Engineering an antiplatelet adhesion layer on an electrospun scaffold using porcine endothelial progenitor cells.

Zhang X, Xu Y, Thomas V, Bellis SL, Vohra YK
Journal of biomedical materials research. Part A 2011 May;97(2):145-51

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Differentiation of human mesenchymal stem cells: the potential mechanism for estrogen-induced preferential osteoblast versus adipocyte differentiation.

Zhao JW, Gao ZL, Mei H, Li YL, Wang Y
The American journal of the medical sciences 2011 Jun;341(6):460-8

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The differences between the endothelial area marked with CD31 and CD105 in colorectal carcinomas by computer-assisted morphometrical analysis.

Jung I, Gurzu S, Raica M, Cîmpean AM, Szentirmay Z
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2009;50(2):239-43

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The expression of cytoskeleton regulatory protein Mena in colorectal lesions.

Gurzu S, Jung I, Prantner I, Ember I, Pávai Z, Mezei T
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2008;49(3):345-9

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The angiogenesis in colorectal carcinomas with and without lymph node metastases.

Gurzu S, Jung J, Azamfirei L, Mezei T, Cîmpean AM, Szentirmay Z
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2008;49(2):149-52

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Isolation and culture of different epidermal and dermal cell types from human scalp suitable for the development of a therapeutical cell spray.

Schlabe J, Johnen C, Schwartlander R, Moser V, Hartmann B, Gerlach JC, Küntscher MV
Burns : journal of the International Society for Burn Injuries 2008 May;34(3):376-84

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The aspects of angiogenesis in anal canal carcinomas compared with that in colorectal carcinomas.

Gurzu S, Jung J, Azamfirei L, Mezei T, Cîmpean AM
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2007;48(4):349-53

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Significance of neo-angiogenesis and immuno-surveillance cells in squamous cell carcinoma of the tongue.

Alkhabuli JO
The Libyan journal of medicine 2007 Mar 1;2(1):30-9

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Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver.

Duret C, Gerbal-Chaloin S, Ramos J, Fabre JM, Jacquet E, Navarro F, Blanc P, Sa-Cunha A, Maurel P, Daujat-Chavanieu M
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1779-90

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Expression of vascular endothelial growth factor in early cutaneous melanocytic lesion progression.

Einspahr JG, Thomas TL, Saboda K, Nickolof BJ, Warneke J, Curiel-Lewandrowski C, Ranger-Moore J, Duckett L, Bangert J, Fruehauf JP, Alberts DS
Cancer 2007 Dec 1;110(11):2519-27

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Correlation between CD105 expression and postoperative recurrence and metastasis of hepatocellular carcinoma.

Yang LY, Lu WQ, Huang GW, Wang W
BMC cancer 2006 May 2;6:110

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Expression of NOS and HIF-1alpha in human colorectal carcinoma and implication in tumor angiogenesis.

Yu JX, Cui L, Zhang QY, Chen H, Ji P, Wei HJ, Ma HY
World journal of gastroenterology 2006 Aug 7;12(29):4660-4

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Expression of NOS and HIF-1alpha in human colorectal carcinoma and implication in tumor angiogenesis.

Yu JX, Cui L, Zhang QY, Chen H, Ji P, Wei HJ, Ma HY
World journal of gastroenterology 2006 Aug 7;12(29):4660-4

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[Mesenchymal stem cells transduced by PLEGFP-N1 retroviral vector maintain their biological features and differentiation].

He X, Li YL, Wang XR, Guo X, Niu Y
Chinese medical journal 2005 Oct 20;118(20):1728-34

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Cell fusion-independent differentiation of neural stem cells to the endothelial lineage.

Wurmser AE, Nakashima K, Summers RG, Toni N, D'Amour KA, Lie DC, Gage FH
Nature 2004 Jul 15;430(6997):350-6

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of CD105 using CD105 Monoclonal Antibody (Product # MA5-11854) on HUVEC Cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with CD105 antibody using peroxidase-conjugate and AEC chromogen. Note staining of endothelial cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD105/endoglin (green) showing staining in the in the membrane of human uterus tissue (right) compared to a negative control without primary antibody (left). Formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Tissue was probed with a CD105/endoglin monoclonal antibody (Product # MA5-11854) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Tissue was washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD105 in U937 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD105 monoclonal antibody (Product # MA5-11854) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of mesenchymal stem cell marker CD105 (green) in human mesenchymal stem cells CY-2 derived from iPSCs. The cells were fixed with 4% Paraformaldehyde for 30 mins and blocked with blocking buffer for 1 hour at room temperature. Cells were stained with CD105 mouse monoclonal antibody (Product # MA5-11854) at a dilution of 1:100 in staining buffer overnight at 4°C, and then incubated with secondary antibody AlexaFluor 488 donkey anti-mouse (Product # A-21202) at a dilution of 1:250 for 1 hour at room temperature. Nuclear (blue) were stained with NucBlue Fixed Cell Stain (Product # R37606). Images were taken at 20X magnification. Data courtesy of Dr. Wei Zheng's lab.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP-MS enrichment of CD105/ENG (LFQ intensity): CD105/ENG was enriched 734-fold from SK MEL 5 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and ENG antibody (Product # MA5-11854). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity.