PA5-27205

antibody from Invitrogen Antibodies

Targeting: ENG CD105, END, HHT1, ORW, ORW1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27205

Provider

Invitrogen Antibodies

Product name

CD105 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD105/Endoglin using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD105/Endoglin polyclonal antibody (Product # PA5-27205) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD105 in whole cell extract (30 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with CD105 Polyclonal antibody (Product # PA5-27205) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD105 Polyclonal Antibody (Product # PA5-27205). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD105 Polyclonal Antibody (Product # PA5-27205) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD105 Polyclonal Antibody detects CD105 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. CD105 stained by CD105 Polyclonal Antibody (Product # PA5-27205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using CD105 (Product # PA5-27205) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.