Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-29555 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD105 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, HCT116. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD105/Endoglin using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD105/Endoglin polyclonal antibody (Product # PA5-29555) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD105 in whole cell extract (30 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with CD105 Polyclonal antibody (Product # PA5-29555) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD105 Polyclonal Antibody (Product # PA5-29555). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD105 Polyclonal Antibody (Product # PA5-29555) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD105 Polyclonal Antibody detects CD105 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. CD105 stained by CD105 Polyclonal Antibody (Product # PA5-29555) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD105 Polyclonal Antibody detects CD105 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. CD105 stained by CD105 Polyclonal Antibody (Product # PA5-29555) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD105 Polyclonal Antibody detects CD105 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. CD105 stained by CD105 Polyclonal Antibody (Product # PA5-29555) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.