DP3517

antibody from Acris Antibodies GmbH

Targeting: ENG CD105, END, HHT1, ORW, ORW1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DP3517

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DP3517, RRID:AB_974108

Product name

anti CD105 / Endoglin

Antibody type

Polyclonal

Antigen

Recombinant Human soluble CD105/Endoglin (aa. 22 (Glu) to 586 (Leu)) derived from Insect Cells (Cat.-No DA3523X).

Reactivity

Human

Host

Rabbit

Vial size

0.2 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western analysis of recombinant human (Cat.-No DA3523) and human (Cat.-No DA3522) soluble CD105 using an anti-human CD105 antibody (Cat.-No DP3517/DP3517S) directed against recombinant human and mouse soluble CD105 produced in insect cells. The SDS-PAGE was run under reducing conditions. There is a strong cross reaction between human and mouse visible.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Double staining of human colon and colon carcinoma tissue with Endoglin (Cat.-No DP3517) / CD31. The experiments were perfomed by Dr. Ulrike Fiedler and Stefanie Koidel, Dept. of Vascular Biology and Angiogenesis Research, Tumor Biology Center, Breisacher Str. 117, D-79106 Freiburg, Germany

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

FACS analysis with antibodies against murine CD105/Endoglin in C57B16 cells

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

FACS analysis with primary human umbilical vein endothelial cells (HUVEC).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).