Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [5]
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Validation data
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- Product number
- NB110-58718 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-58718, RRID:AB_920830
- Product name
- Mouse Monoclonal Endoglin/CD105 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody (clone MEM-229) recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow
- Reactivity
- Human, Canine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Bone marrow donor selection and characterization of MSCs is critical for pre-clinical and clinical cell dose production.
Short-term hypoxic preconditioning promotes prevascularization in 3D bioprinted bone constructs with stromal vascular fraction derived cells.
Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs.
Trivedi A, Miyazawa B, Gibb S, Valanoski K, Vivona L, Lin M, Potter D, Stone M, Norris PJ, Murphy J, Smith S, Schreiber M, Pati S
Journal of translational medicine 2019 Apr 17;17(1):128
Journal of translational medicine 2019 Apr 17;17(1):128
Short-term hypoxic preconditioning promotes prevascularization in 3D bioprinted bone constructs with stromal vascular fraction derived cells.
Kuss MA, Harms R, Wu S, Wang Y, Untrauer JB, Carlson MA, Duan B
RSC advances 2017 Jun 5;7(47):29312-29320
RSC advances 2017 Jun 5;7(47):29312-29320
Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs.
Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P
Physiological research 2009;58(6):885-93
Physiological research 2009;58(6):885-93
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (MEM-229) NB110-58718PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Analysis of the APC conjugate of this antibody. A cell surface stain was performed on THP-1 cells with Endoglin antibody NB110-58718APC (blue) along with a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PerCP-CyTM5.5.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) biotin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PE.