NB100-65956

antibody from Novus Biologicals

Targeting: KLRK1 CD314, D12S2489E, KLR, NKG2-D, NKG2D

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Functional assay (Data presented on provider website)

Antibody Data

Product number

NB100-65956

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-65956, RRID:AB_960833

Product name

Mouse Monoclonal NKG2D/CD314 Antibody

Antibody type

Monoclonal

Description

Protein A purified. NB100-65956 is specific for CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding to CD314 results in NK cell activation and potent costimulation of effector T cells. Clone 1D11 is reported to inhibit T cell recognition of MICA (1,6).

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.25 mg

Concentration

1.0 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Hypoxia-induced shedding of MICA and HIF1A-mediated immune escape of pancreatic cancer cells from NK cells: role of circ_0000977/miR-153 axis.

Ou ZL, Luo Z, Wei W, Liang S, Gao TL, Lu YB
RNA biology 2019 Nov;16(11):1592-1603

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Quantitative and functional analysis of CD69⁺ T regulatory lymphocytes in patients with periodontal disease.

Vitales-Noyola M, Martínez-Martínez R, Loyola-Rodríguez JP, Baranda L, Niño-Moreno P, González-Amaro R
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2017 Aug;46(7):549-557

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Detection and significance of cytotoxic cell subsets in biopsies of HCV-infected human livers.

Mozer-Lisewska I, Mania A, Kowala-Piaskowska A, Kluk A, Samara H, Pauli A, Żeromski J
Archivum immunologiae et therapiae experimentalis 2014 Apr;62(2):153-60

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD314:Low Endotoxin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Analysis using the Biotin conjugate of NB100-65956. Staining of Human peripheral blood lymphocytes with biotinylated 1D11, followed by Sav-PE.