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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA1-41285 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAF7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: antigen standard for TRAF7 (transient overexpression lysate).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRAF7 in human Hela lysate in the A) absence and B) presence of immunizing peptide using a TRAF7 polyclonal antibody (Product # PA1-41285) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRAF7 in Huvec, MCF7, U2-OS, A431, SHSY-5Y and HeLa cells. Samples were incubated in TRAF7 polyclonal antibody (Product # PA1-41285) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. Separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.
