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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-2462 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2462, RRID:AB_10002841
- Product name
- Goat Polyclonal Phosphodiesterase 4D Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antiobody is expected to recognize all reported isoforms.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells.
The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target.
Bazhin AV, Kahnert S, Kimpfler S, Schadendorf D, Umansky V
Molecular immunology 2010 Jan;47(4):678-84
Molecular immunology 2010 Jan;47(4):678-84
The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target.
Houslay MD
Molecular pharmacology 2005 Sep;68(3):563-7
Molecular pharmacology 2005 Sep;68(3):563-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - HEK293 overexpressing Human PDE4D2 and probed with (mock transfection in first lane).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Phosphodiesterase 4D Antibody [NB100-2462] - (1ug/ml) of COS cell lysates (25ug protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NB100-2462] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
